Williams′ Medium E

Background

Williams′ Medium E is a modification version of Williams′ Medium. Although it is like MEM, there is differences in glucose as well as amino acid contents.  This media was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been shown that it is suited for longer-term adult primary cell culture.

PurMa™ William’s E Medium is

• Suited for long-term culture.
• Enriched in amino acids.
• Also, double the glucose compared to the original formulation.
• Moreover, contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione as well as lipid methyl linoleate

Moreover, PurMa™ William’s E Medium also contains:

• Low level of glucose (2.00 g/ liter). PurMa Biologics manufacturers the high glucose (4.5 g/L) version of this media as well (Cat #: P3S029880)
• Although the standard formulation contains 2.2 g/L Sodium bicarbonate, PurMa Biologics manufacturers the high sodium bicarbonate (3.7 g/L) Williams′ Medium E as well (Cat #: P3S032880)
• 2 mM L-glutamine (292 mg/L)’

Moreover, PurMa Biologics makes 112 types of William’s E Medium which is certainly the most comprehensive catalog of media.

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. our company uses %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

Application of William’s E-Medium

Additionally, This media has elements supporting the long-term culturing of primary cells. It utilizes a bicarbonate buffering system. it has been used for primary as well as commercial cell lines including primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas as well and hybridoma cells.

Formulation:

The complete formulation is available in the formulation tab as well here: William’s E Medium Formulation