PurMa Biologics Manufactures Cryoprotective Media without DMSO or other penetrating agent. To emphasize, DMSO-free cryoprotective needs to apply only for certain cell lines which are damaged or poorly performing in presence of DMSO or other penetrating agents.

limitations and disadvantages of DMSO

1. Basically, it has a broad toxicity.
2. Additionally, it modifies the expression of cells such an NK and T cells
3. It alters the mRNA level of the de novo DNA methyltransferase DNMT3A. this therefore, accompanies by hyper- or hypo-methylation of many genetic loci.
4. Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.
5. Damages the mitochondria in certain cell culture models such as astrocytes.
6. Negatively impacs cellular membrane/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.
7. Besides, it Induces unwanted stem cell differentiation.
8. Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated sage of DMSO even at sub-toxic levels.
9. Afterwards, it is necessary to remove DMSO by intensive washing. That in turns results in cell sensitivity to osmotic/mechanical stresses occurring. This is a costly- and time-consuming process during freezing and thawing process and moreover leads to a significant loss of cell number.

We produce cryoprotective media for :

• Serum Free condition,  DMSO Free
• Stem Cells ,  DMSO Free
• Whole Tissue ; DMSO Free
• Hybridoma cells ; DMSO Free