Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer

Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-MEM-α-N needs 2-5% FBS and provides more consistency.

Condition

Format

Size

Clear

SKU: N/A Categories: MEM α-Modification with Nucleosides in Hanks’ Buffer, Cell Culture Media Tags: Advanced MEM Alpha, Recombinant Insulin, Human Transferrin, serum reduction, L-Alanyl-L-Glutamine, Advanced, cell culture, Nucleosides, Hanks’ Buffer, MEM-α, HBSS

Description

Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS)

Background

PurMa^TM Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS) contains Nucleosides as well as components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-MEM-α-N, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Advanced MEM-α-N in HBSS Contains:

includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
A-MEM-α-N incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

PurMa^TMHuman Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
PurMa^TM Insulin Recombinant
Trace of other elements which mimic the elements in fetal bovine serum.

PurMa^TM Advanced MEM-α with nucleoside (A-MEM-α-N) is modified from Advanced MEM-α with the following ingredients:

Ribonucleosides
Deoxyribonucleosides

Consequently, Advanced MEM-α with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α-N (CD- A-MEM-α-N) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Adnaced MEM Alpha with Nucleoside in HBSS buffer Formulation

References

A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt E, Eriksson M. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026
The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

Additional information

Condition	A-MEM-α-N (HBSS) Standard Formulation, A-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine, A-MEM-α-N (HBSS) w/o Phenol Red, A-MEM-α-N (HBSS) w/o Sodium Bicarbonate, A-MEM-α-N (HBSS) w/o Glucose, A-MEM-α-N (HBSS) w/o Lipoic Acid, A-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine, w/o Sodium Bicarbonate, A-MEM-α-N (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), A-MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), A-MEM-α-N (HBSS) w/o Sodium Pyruvate, A-MEM-α-N (HBSS) High Sodium Bicarbonate (0.85 g/L), A-MEM-α-N (HBSS) High Glucose (4.5 g/L), A-MEM-α-N (HBSS) With 15 mM HEPES (3.6 g/L), w/o Glucose, A-MEM-α-N (HBSS) With 25 mM HEPES (5.9 g/L), w/o Glucose, A-MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), A-MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), A-MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), A-MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L)
Format	Liquid, Powder
Size	10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

Advanced-MEM-α-N with Hanks’ Buffer (HBSS) Formulation

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