Kaighn’s Modification of Ham’s F-12K
Kaighn’s Modification of Ham’s F-12K Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) worked properly for primary cells such as human oocytes and hepatocytes. This medium also performed well for murine and chicken liver cells.
it contains a variety of components not found in older media. That includes putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These compensated for supplemental serum. This media still needs the addition of 10 % FBS . this medium Medium contains no proteins or growth factors and therefore needs FBS. F-12K Medium also contains: Low level of glucose (1.26 g/ liter), Sodium bicarbonate (2.5 g/L) (if
PurMa Biologics also offers F-12K Medium with low sodium bicarbonate (1.5 g/L) (Cat # P3S057108) as well as high sodium bicarbonate (3.7g/L) (Cat # P3S032108)
Kaighn’s Modification of Ham’s F-12K Medium also contains 2 mM L-glutamine (292 mg/L)’, A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
PurMa Biologics manufactures 96 types of Ham’s F-12K which accommodate any form of this medium.
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- Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium
PurMa™ Chemically Defined Kaighn’s Modification of Ham’s F-12 Medium (CD-Ham’s F-12K)
- PurMa™ Chemically Defined Kaighn’s Modification of Hams F12 Medium (CD-Ham’s F12K) is a 100% synthetic. It is FBS-free, animal-component–free, chemically defined Ham’s F-12K medium. Additionally, it replaces serum with a proprietary synthetic cocktail of growth factors, hormones, and nutrients. This ensures batch-to-batch consistency and regulatory-friendly workflows. Moreover, CD-Ham’s F-12K contains L‑alanyl‑L‑glutamine instead of L‑glutamine. This provides improved stability, longer shelf…
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- Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
- Price range: $27.97 through $605.10
- A high-performance DMEM/F-12 formulation engineered for consistent mammalian cell culture.Enriched with 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, 30 mM NaHCO3, high glucose, non-essential amino acids, and phenol redIdeal for MDCK, glial cells, fibroblasts, and human endothelial cultures
- Select options This product has multiple variants. The options may be chosen on the product page
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- Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium
PurMa™ Kaighn’s Modification of Ham’s F-12-K Medium
- Price range: $21.05 through $518.27
- PurMa™ Kaighn's Modification of Ham's F-12-K Medium is a specialized cell culture medium designed to enhance the growth and maintenance of various cell types, providing essential nutrients and optimized conditions for cellular proliferation and experimentation.
- Select options This product has multiple variants. The options may be chosen on the product page
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- Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium
PurMa™ Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K-MAX)
- Price range: $28.82 through $608.99
- PurMa™ F-12K-MAX, an advanced formulation of Ham's F-12K L‑Alanyl‑L‑Glutamine, enhances the traditional Kaighn’s F-12 medium by incorporating 4 mM L‑alanyl‑L‑glutamine. This modification ensures superior stability and significantly reduces ammonia formation while improving the medium's buffering capacity. Additionally, PurMa™ F-12K-MAX offers an extended shelf life and is specifically optimized to support primary and sensitive mammalian cell cultures effectively.
- Select options This product has multiple variants. The options may be chosen on the product page



