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Whole Tissue Cryoprotective Media (WTCM-D) with DMSO
Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. This inevitably, cause volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO, preserve tissues during rapid freezing. This remarkably promotes formation of amorphous ice ( rather than crystal ice).
Description
Background
Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. This inevitably causes volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO preserves tissues during rapid freezing. This remarkably promotes formation of amorphous ice ( rather than crystal ice).
PurMa™ WTCM-D has particularly overcome several challenges
- Liquid nitrogen has a low specific heat constant. When it contacts warm tissues or OCT it boils and forms a vapor barrier and acts as an insulator. As a result, the inner core of the tissue sample freezes slowly and unevenly. This is especially more challenging when the tissue sample is large. The cryoprotective agents in PurMa™ WTCM-D prevent this from happening by penetrating rapidly inside the tissue.
- PurMa™ WTCM-D acts regardless of the size of the tissue. PurMa™ WTCM has high thermal conductivity. This, therefore, allows for rapid and efficient cryoprotection using liquid nitrogen.
- PurMaTM WTCM contains ingredients which make it suitable for the preservation of rigid tissues.
Special Features
- Ready-to-Use.
- PurMaTM WTCM provides much higher cell viability after short/long-term cryoprotection,
- PurMaTM WTCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
- Preserves morphological appearance as a standard factor of cell recovery.
- Tested for pH, osmolality, sterility, and endotoxin.
- Each Lot of PurMaTM WTCM is tested for pathogens and sterility. We evaluate each Lot as well for several criteria, including survival (by Trypan Blue method), viability & performance (by MTT Test), and the recovery rate by Alamar Blue test.
- PurMaTM WTCM has been tested successfully for several commonly used tissue including rodent heart, brain, and kidney.
Guideline
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines
References
- Diffusion and distribution of dimethyl sulphoxide in the isolated guinea-pig taenia coli. Elford BC. J Physiol. 1970 Jul;209(1):187-208. doi: 10.1113/jphysiol.1970.sp009162.
- Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants. Isachenko et al. Clin Lab. 2015;61(3-4):415-20. doi: 10.7754/clin.lab.2014.140919. PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is Cryoprotective Media?
Additional information
| Size | 1 x 10 ml, 1 x 100 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
it is reliable vendor!
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I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
Frequently asked questions
Should I choose the DMSO or DMSO free formulation?
DMSO is the standard cryoprotectant and gives the best recovery for most lines. Choose DMSO free where DMSO would interfere with the application, for example in differentiation work or certain clinical and hybridoma protocols where residual DMSO is a problem.
What freezing rate should I use?
Approximately minus 1 degree C per minute to around minus 80, using a controlled rate cooler or an insulated freezing container, before transfer to liquid nitrogen. Faster cooling causes intracellular ice and slower cooling causes osmotic damage.
How should vials be stored long term?
In the vapour phase of liquid nitrogen, below minus 150 degrees C. Storage in a minus 80 freezer is acceptable only for weeks, not years, because viability declines steadily above minus 130.
How should I thaw cells frozen in Whole Tissue Cryoprotective Media (WTCM-D) with DMSO?
Thaw rapidly in a 37 degree C bath until only a small ice crystal remains, then dilute promptly into pre warmed medium. DMSO is toxic to cells at room temperature, so the interval between thaw and dilution should be as short as possible.
How should the cryoprotective media itself be stored?
Store as stated on the Certificate of Analysis, protected from light. Do not refreeze after thawing the media, and use within the period stated for the lot.
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