Description

Whole Tissue Cryoprotective Media (WTCM-D) with DMSO

Background

Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells.  This inevitably, cause volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO, preserve tissues during rapid freezing. This remarkably promotes  formation of amorphous ice ( rather than crystal ice).

PurMa™ WTCM-D  has particularly overcome several challenges

1. Liquid nitrogen has a low specific heat constant.  When it contacts warm tissues or OCT it boils and forms a vapor barrier and acts as an insulator. As a result, the inner core of the tissue sample freezes slowly and unevenly. This is especially more challenging when the tissue sample is large. The cryoprotective agents in PurMa™ WTCM-D precents this to happen by penetrating rapidly inside the tissue.
2. PurMa™ WTCM-D acts regardless of the size of the tissue. PurMa™ WTCM has high thermal conductivity. This, therefore, can allow liquid nitrogen for rapid and efficient cryoprotection.
3. PurMa^TM WTCM contains ingredients which makes it suitable for the preservation of rigid tissues.

Special Features

• Ready-to-Use.
• PurMa^TM WTCM provides much higher cell viability after short/long-term cryoprotection,
• PurMa^TM WTCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
• Preserves morphological appearance as a standard factor of cell recovery.
• Tested for pH, osmolality, sterility, and endotoxin.
• Each Lot of PurMa^TM WTCM is tested for pathogens and sterility. We evaluate each Lot as well for several criteria. Including survival (by Trypan Blue method), viability & performance (by MTT Test), and the recovery rate by Alamar Blue test.
• PurMa^TM WTCM has been tested successfully for several commonly used tissue including rodent heart, brain, and kidney.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

1. Diffusion and distribution of dimethyl sulphoxide in the isolated guinea-pig taenia coli. Elford BC. J Physiol. 1970 Jul;209(1):187-208. doi: 10.1113/jphysiol.1970.sp009162.
2. Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants. Isachenko et al. Clin Lab. 2015;61(3-4):415-20. doi: 10.7754/clin.lab.2014.140919. PMID: