- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Ultra-Pure DEPC Treated Nuclease Free Water
PurMa™ DEPC Treated Ultra-Pure Nuclease Free Water is suitable very sophisticated molecular biology applications. moreover, it has been DEPC Treated DNase/RNase Free.
Description
Background
PurMa™ Ultra-Pure Nuclease Free Water is suitable for most molecular biology applications.
Special Feature
- Purified by Reverse Osmosis (18.2 ΩM/cm2).
- DEPC Treated DNase/RNase Free.
- Autoclaved
- Double-Distilled.
- Sterilized by 0.1 µm filters.
- Analyzed for conductivity, resistancy.
- Sterilized by 0.1 µm filters.
- Packed under N2
PurMa™ Ultra-Pure Nuclease Free Water is suitable for high accuracy molecular biology studies as well as analytical analysis applications such as:
- mRNA & Total RNA Isolation.
- Mass spectrometry and applications demand highly strict control.
- Electrophoresis, cloning, PCR, RNA, and DNA isolation.
- Chromosome analysis or karyotyping.
- Northern and southern blotting.
- Evaluated for proteases.
- Evaluated for phosphatases.
References
- Comparison of Milli-Q PF Plus water to DEPC-treated water in the preparation and analysis of RNA. Huang et al. 1995 Oct;19(4):656-61.
- Isolation of Poly(A)+ Messenger RNA Using Magnetic Oligo(dT) Beads. Green et al. Cold Spring Harb Protoc. 2019 Oct 1;2019(10).
| General specifications | |
| Parameter | Specification |
| Grade | Ultra-pure Molecular Biology |
| Appearance | Clear |
| pH | 5-7 |
| Osmolality | 275-360 mOsm/L |
| Filtration | Sterile filtered using 0.1 µm filter |
| Indicator | NA |
| Conductivity (Measured) | 0.056 µs/cm2 |
| Resistancy (Calculated) | 18.2 Ωm |
| Shipping Condition | Ambient |
| Refractive index | n20/D 1.34 (lit.) |
| Molecular Biology Parameters | |
| DNases & RNases | NOT Detected |
| proteases | NOT Detected |
| phosphatases | NOT Detected |
| Pathogens Evaluation | |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Mold & Fungi/Yeast | Negative |
| Bacteria | Negative |
| Sodium pyruvate | NA |
Additional information
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is Ultra-Pure DEPC Treated Nuclease Free Water endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
Related products
-
- Sale!
- Trypsin EDTA and EDTA-Free, Trypsin With EDTA
Trypsin-EDTA 0.25%
- Price range: $13.99 through $412.88
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Cell Culture Suited Water and Water Treatments
Water Bath Treatment (500X)
- Price range: $198.92 through $699.26
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Water and Water Treatments
CO2 Incubator Treatment (100X)
- Price range: $189.56 through $744.36
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews