Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)
Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-IMDM needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-IMDM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced IMDM Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-IMDM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced-IMDM (CD-A-IMDM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation of Advanced IMDM: Advanced IMDM Formulation
References
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, et al. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
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azadi@live.ca –
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Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
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lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
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michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
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jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
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hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
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jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
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tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
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Fitzkr@gmail.com –
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Always great quality, fast processing and shipping.
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rohan-varshney@ouhsc.edu –
Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
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