Phosphate Buffered Saline (PBS)

Description

Background

Phosphate Buffered Saline (PBS) is a pH adjusted buffer solution used in biological research.  Moreover, it is a water-soluble blend of ultra pure grade phosphate buffers and saline solutions. Additionally, PurMa Biologics manufactures PBS for regular uses as well as for cell culture purposes. This especially applies to protocols requiring use of consistent conditions such as crosslinking, biotinylation and fluorescent labeling reactions. The mentioned applications certainly require an amine-free buffer.

General Feature

• PurMa Biologics manufactures this buffer in two pH: 7.4 ± 0.01 and 7.2 ± 0.01 at 25°C.
• We rigorously sterilize it by 0.22µm membrane.
• Additionally, it is a cost-effective balanced salt solution used for a variety of biochemical applications.
• PurMa Biologics packs all our buffer under N_2, as a result, the pH won’t change over time before opening the bottle.

Specific Feature

• Cell Culture Suited
• We deplete  Mg²⁺ and Ca²⁺ ions
• Our manufacturing process removes  RNAse /DNase
• Free of Proteases.
• PurMa Biologics  rigorously test for contaminating,  nonspecific endonuclease, exonuclease, as well as  RNase activity.

For complete formulation, Click here, as well as formulation tab:PBS Formulation

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

References:

1.  Plaque formation and isolation of pure lines with poliomyelitis viruses. Dulbecco et al., J Exp Med.; 1954 Jan 31 99(2): 167–182. doi: 10.1084/jem.99.2.167. PMID: 13130792.
2. Modulation of the random migration of human platelets. Valone et al. J Clin Invest. 1974 Nov;54(5):1100-6. doi: 10.1172/JCI107854. PMID: 4608469

 

Learn more: What Is PBS?