PurMaFectin™ 293T System
PurMaFectin™ 293T System provide exceptional transient and stable transfection with over 95% efficacy. Moreover, it offers an exceptional result for commercial cell lines including HEK 293, COS1, COS9 cells and adhesive cell lines. It completes the procedure in 35-40 minutes.
Description
Background
PurMaFectin™ 293T System provide exceptional transient and stable transfection with over 95% efficacy. Moreover, it offers an exceptional result for commercial cell lines including HEK 293, COS1, COS9 cells and adhesive cell lines. It completes the procedure in 35-40 minutes.
PurMaFectin™ 293T System Special Features
- It is easy to use.
- PurMaFectin™ 293T System does not cause cell toxicity.
- Provides consistent and reproducible transfection.
- More importantly, This transfection reagent suites both transient and stable transfection.
- PurMaFectin™ 293T protects DNA from lysosomal degradation and facilitate efficient plasmid delivery into eukaryotic nucleus.
- Also, it provides effective, reproducibility, and affordable benefits for scientific research.
Brief Guideline
Below, you can find some brief guidelines. For detailed protocol, click here:PurMaFectin293 User Manual
- For the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. Additionally, we strongly recommend starting the seeding by counting trypsinized cells so you maintain high consistency among your experiments.
- Different cell types react differently to the number of passages when they are used for transfection. To minimize the impact of passage on efficiency of transfection, either use the cells with the same passage number or use at least two different concentrations of transfection reagent as control in new transfect experiments before you optimize the condition.
- For HeLa cells, high-level transfection requires the presence of serum in the medium during transfection. The amount of required serum depends on sequence and the length of plasmid. The exact level of serum should be determined empirically.
- Furthermore, we deplete endotoxin from our transfection reagent. The endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. To deplete the endotoxin, we use PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). In this proprietary kit, Sepharose 6B covalently conjugates to an endotoxin-specific absorbent. The mentioned procedure will end with passing the reagent through 0.1µm filters 3X (included in the kit). The mentioned eliminates the residual endotoxin.
References
- A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization. Ooi et al. Front Physiol. 2016 Jul 19; 7:300. doi: 10.3389/fphys .2016 .00300. eCollection 2016. PMID: 27486406.
- Gene delivery in adherent and suspension cells using the combined physical methods. Kardani et al. Cytotechnology. 2022 Apr;74(2):245-257. doi: 10.1007/s10616-022-00524-4. Epub 2022 Feb 3. PMID: 35464169.
| Parameter | Specification |
| Appearance | clear liquid, Red liquid |
| pH | 7.4 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | NA |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Dry Ice |
Additional information
| Size | 1 x 1 ml, 5 x 1 ml |
|---|
18 reviews for PurMaFectin™ 293T System
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5
hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
Have you purchased: Yes
Time of submission: 17-Mar-2025 12:09:58
Ease of placing the order: 5/5
jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
Have you purchased: Yes
Time of submission: 17-Mar-2025 14:22:58
Ease of placing the order: 4/5
tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
Have you purchased: Yes
Time of submission: 18-Mar-2025 10:01:50
Ease of placing the order: 5/5
Fitzkr@gmail.com –
Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
Always great quality, fast processing and shipping.
We are totally satisfied.
Have you purchased: Yes
Time of submission: 22-Mar-2025 10:47:00
Ease of placing the order: 5/5
vmadka@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:02:40
Ease of placing the order: 4/5
Anupam-Mandal@ouhsc.edu –
Review: Excellent
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:05:43
Ease of placing the order: 5/5
rohan-varshney@ouhsc.edu –
Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:06:38
Ease of placing the order: 5/5