PurMaFectin™ Ultra Transfection Reagent

Description

Background

PurMa Biologics manufactures PurMaFectin™ Ultra Transfection Reagent with over 97% efficacy. Also, this reagent performs in a non-liposomal manner. Our Ultra Transfection Reagent provides consistent/reproducible transfection with ignorable cytotoxicity. PurMaFectin™ Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian/ eukaryotic nucleus.

Special Features

For complete protocol, click here: PurMaFectin™ Ultra_ Use Manual

• • Basically, the entire procedure with PurMaFectin™ Ultra is completed within 30 minutes.
  • Furthermore, for the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. We strongly recommend starting the seeding by counting the trypsinized cells so there will be high consistency among your experiments.
  • Additionally, different cell types react differently to the number of passages when they are used for transfection. So, to minimize the impact of passage on efficiency of transfection, use the cells with the same passage number, or alternatively, apply at least two different concentrations of transfection reagent as control in new transfect experiments before the condition is optimized.
  • We deplete endotoxin from our transfection reagent. The  endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. To deplete the endotoxin, we use PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). In this proprietary kit, Sepharose 6B covalently conjugates to  an endotoxin-specific absorbent. The mentioned procedure will end with passing the reagent through 0.1µm filters 3X (included in the kit). The mentioned procedure eliminates the residual endotoxin.

References

1. Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents. Rahimi et al. 2018;119(11):701-705. doi: 10.4149/BLL_2018_125. PMID: 30686003.
2. Transfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells. Zhang et al. Biotechniques. 1996 Nov;21(5):940-2, 944-5. doi: 10.2144/96215pf02. PMID: 8922638.