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Stem Cell Cryoprotective Media (SCCM-D) With DMSODMSO

Stem Cell Cryoprotective Media (SCCM-D) With DMSO

(18 customer reviews)

PurMa Biologics is the leading company in Manufacturing Cryoprotective Media. Moreover, PurMa™ Stem Cell Cryoprotective Media With DMSO is the next generation Cryoprotective Media . It considerably limits the cell damage during the freezing and ensures the recovery, survival, and viability as well as functionality after thawing.

SKU: N/A Categories: Cryoprotective Media, Cryoprotective media With DMSO Tags: Cryoprotective Media, Stem Cells, With DMSO

Description

Background

Certainly, there has been a development in the cryopreservation of several stem cells. Especially in  hematopoietic mesenchymal stromal and induced pluripotent stem cells.  Post‑thaw cell death remains, however a considerable problem in stem cell technology. Moreover, Stem Cell Cryoprotective Media (SCCM-D) With DMSO considerably limits several current issues. Those includes  the cell damage during the freezing and ensures the recovery, survival, and viability as well as functionality after thawing.

Why Cryopreservation

During cryopreservation,  cooling aqueous solutions shifts below the equilibrium freezing point. This will subsequently result in the formation of ice crystals in the extracellular media. This  will cause a decrease in concentration of water which has two key consequences:

  1. Generally, osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
  2. Consequently, Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.

With decades of supremacy in stem cell biology, biochemistry, and cell culture, PurMa Biologics has developed a next generation cryoprotective media specialized for delicate stem cell culture technology. PurMa™ SCCM-D contains DMSO and gives the best preservation for stem cells, particularly proven successfully on hematopoietic, mesenchymal stromal, and induced pluripotent stem cells.

Special Features

  • Ready-to-Use.
  • Moreover, PurMa™ SCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.
  • PurMa™ SCCM-D With DMSO provides much higher cell viability after short/long-term cryoprotection, (66% recovery rate)
  • PurMaTM SCCM-D Prevents the formation of crystals in extracellular space. This  consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
  • Preserves morphological appearance as a standard factor of cell recovery.
  • We Test PurMa™ SCCM-D  for pH, osmolality, sterility, and endotoxin.
  • Each Lot of PurMaTM SCCM-D goes through several important tests. Those are  a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.
  • PurMaTM SCCM-D has been tested successfully for several commonly used stem cells. The mostly used are  including hematopoietic, mesenchymal stromal/stem, and induced pluripotent stem cells.
  • Suitable for clinical applications.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

  1. Long-term preservation of permanent human hematopoietic cell lines. Mizrahi et al. J Med. 1971; 2(6) :380-6.
  2. Survival of human bone marrow progenitor cells after freezing: improved detection in the colony-formation assay. Schlunk et al. 1981 Apr;18(2):111-8. doi: 10.1016/0011-2240(81)90082-1
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Size

1 x 10 ml, 1 x 100 ml

18 reviews for Stem Cell Cryoprotective Media (SCCM-D) With DMSO

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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