- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Cell Culture Suited Water
PurMa™ Cell Culture Suited Water is suitable for day-to-day culture experiments. Moreover, we deplete Ca+2 and Mg +2 and vigorously evaluate all our waters for mycoplasma and endotoxins.
Description
Background
PurMa™ Cell Culture Suited Water is suitable for day-to-day culture experiments. Moreover, it has been depleted of Ca+2 and Mg+2.We vigorously evaluate all our waters for mycoplasma and endotoxins.
Special Features
- Purified by Deionization (DI) followed Reverse Osmosis process (18.2 ΩM/cm2).
- Additionally, the trace of heavy metals including Ca+2 and Mg +2 have been depleted. This is, however, performed using ion exchange chromatography resins.
- We also sterilize cell culture grade water by 0.2 µm filters.
- Lastly, we pack it under N2.
References
- A Review on Reverse Osmosis and Nanofiltration Membranes for Water Purification. Yang et al. Polymers (Basel). 2019 Jul 29;11(8):1252.
- Extending the life cycle of reverse osmosis membranes: A review. Coutinho de Paula et al. Waste Manag Res. 2017 May;35(5):456-470. Epub 2017 Jan 18.
| General specifications | |
| Parameter | Specification |
| Grade | Cell Culture Suited |
| Appearance | Clear |
| pH | 7.0 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Purification Method | Sterile filtered using 0.22 µm filter |
| Indicator | NA |
| Conductivity (Measured) | 0.056 µs/cm2 |
| Resistancy (Calculated) | 18.2 Ωm |
| Shipping Condition | Ambient |
| Refractive index | n20/D 1.34 (lit.) |
| Molecular Biology Parameters | |
| DNases & RNases | NOT Detected |
| proteases | NOT Detected |
| phosphatases | NOT Detected |
| Pathogens Evaluation | |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Mold & Fungi/Yeast | Negative |
| Bacteria | Negative |
| Sodium pyruvate | NA |
Additional information
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is Cell Culture Suited Water endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
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