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- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
General Molecular Biology Grade Water
PurMa™ Molecular Biology Grade Water manufactures molecular biology water, it suits mosy molecular biology applications. Moreover, it sterilized by 0.22µm filters, free of DNAses & RNAs and finally, not treated with DEPC.
Description
PurMaTM Molecular Biology Grade Water is suitable for most molecular biology applications.
Special Features
- Molecular Biology Grade by Reverse Osmosis (18.2 ΩM/cm2).
- Sterilized by 0.22µm Filters.
- Free of DNAses & RNAses.
- Not treated with DEPC.
- Suitable for most molecular biology applications such as electrophoresis, cloning, PCR, RNA, and DNA isolation.
- Analyzed for conductivity, resistancy.
- Evaluated for proteases.
- Evaluated for phosphatases.
References
- Reverse osmosis and ultrafiltration for recovery and reuse of larval rearing water in Anopheles rabiensis mass production: Effect of water quality on larval development and fitness of emerging adults. Mamai et al. JRL. Acta Trop. 2017 Jun;170:126-133. Epub 2017 Mar 1.
- Isolation of Poly(A)+ Messenger RNA Using Magnetic Oligo(dT) Beads. Green et al. Cold Spring Harb Protoc. 2019 Oct 1;2019(10)
| General specifications | |
| Parameter | Specification |
| Grade | Molecular Biology |
| Appearance | Clear |
| pH | 7.0 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Filtration | Sterile filtered using 0.22 µm filter |
| Indicator | NA |
| Conductivity (Measured) | 0.056 µs/cm2 |
| Resistancy (Calculated) | 18.2 Ωm |
| Shipping Condition | Ambient |
| Refractive index | n20/D 1.34 (lit.) |
| Molecular Biology Parameters | |
| DNases & RNases | NOT Detected |
| proteases | NOT Detected |
| phosphatases | NOT Detected |
| Pathogens Evaluation | |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Mold & Fungi/Yeast | Negative |
| Bacteria | Negative |
| Sodium pyruvate | NA |
Additional information
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is General Molecular Biology Grade Water endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
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