For the greatest results in cell culture and laboratory research, selecting the right media is crucial. Earle’s Balanced Salt Solution, or EBSS for short, is one of the most used research media. That salt solution is made to provide a suitable environment that supports cell visibility and function. Knowing the benefits of EBSS can be helpful for researchers in order to make informed decisions during their research.
Increase Survival of Cells EBSS media
One of the very extensive benefits of EBSS media is that it increases cell survival in a number of experimental setups including a mix of several salts that function in support of the viability of cells within it. Among these are calcium chloride, sodium bicarbonate, and potassium chloride, which are necessary for different functions in the cellular and to maintain osmotic balance.
Maintain Ideal pH
The pH should be maintained at the correct levels so that the cells of the culture are functioning and healthy. Maintaining a stable pH level to contribute to cellular processes is the purpose served by the EBSS medium. This particular property is quite helpful in experiments where cells need to remain viable for quite a while.
Compatible with Different Cell Types
EBSS media is well known for its high compatibility with different cell types. EBSS provides the required support for cell survival from primary cell lines to tissue samples in experiments, which makes EBSS a go-to for various cell cultures when there is a need for a capable medium. The use of EBSS is not limited to its compatibility; it is used for various applications such as cell washing, dilution of cell suspensions, and as a base solution for preparing other complex media. Due to this property of EBSS media, the requirement for multiple salt solutions is reduced.
EBSS vs HBSS
While both of these solutions are Balanced Salt solutions, there are some important differences. HBSS is used for experiments that require simple solutions when there is no need for higher concentrations of calcium and magnesium. However, EBSS provides proper support when high concentrations of these ions are needed for cell functions. Moreover, EBSS contains glucose making it an instant source of energy for cells during short experiments, While HBSS does not have glucose, which limits their efficiency in experiments.
End Note
EBSS media have many advantages for laboratory research, such as maintaining the right pH and proper cell function, and is an essential solution when it comes to cell culture processes. When comparing EBSS vs HBSS, the clear choice is EBSS due to its flexible nature and numerous benefits. Choosing the right media supplier, like PurMa Biologics LLC, is essential for your laboratory experiments as it ensures optimal results.