Description

MEM Essential & Non-Essential Amino Acids Solution (50X)

Background

MEM Essential & Non-Essential Amino Acids Solution (50X) are indeed playing a substantial role in cell culture. Basically, Essential amino acids cannot be synthesized In Vivo,  thus it is extremely important to supplement the media with those to maintain proper cellular growth and expected viability. On the other hands, mammalian cells can synthesize non-essential amino acids (NEAA) through glutaminolysis, glycolysis, or via TCA cycle.

During low concentration of required amino acids in media, cells need to consume more glucose and glutamine that results unexpected amount of bio degradation products, this , indeed acts as a hindrance in proper cellular growth and maintenance. So, adding supplements of amino acids to media reduces biosynthetic burden on cell in vitro, stimulates growth and prolongs the viability of cells.

Moreover, you can find the formulation for PurMa™ MEM Essential Amino Acids: Formulation

additionally, you can find the formulation for PurMa™ MEM Non-Essential Amino Acids:Formulation

PurMa Biologics manufactures the essential and non-essential amino acids using our highest water  , PurMa^TM Ultra-Pure Cell Culture Suited water (Cat#: P3W010101). For more information, click here: PurMa™ Ultra-Pure Cell Culture Suited water

Special Features

• Ready -To-Use.
• Cell Culture Suited.
• Sterilized Using 0. 2 µm Filters.
• Additionally,  they are , Vigorously tested for Mycoplasma and Endotoxin
• and Lastly, all our supplements are Packed Under N2.

References

1. Dietary requirements of “nutritionally non-essential amino acids” by animals and humans. Wu et al. Amino Acids. 2013 Apr;44(4):1107-13. Epub 2012 Dec 18.
2. Ingested non-essential amino acids recruit brain orexin cells to suppress eating in mice. Viskaitis et al. Curr Biol. 2022 Apr 25;32(8):1812-1821.e4. Epub 2022 Mar 21.