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PurMa Essential Amino Acids 50X, 100 mL bottle, from the MEM essential and non-essential amino acids range
  • ✓Proudly made in the USA, manufactured in house
  • ✓Certificate of Analysis available for every lot
  • ✓Backed by decades of cell culture and protein biochemistry expertise
  • ✓Bulk pricing, purchase orders, and custom formulations welcome
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SKU: Select options below to see it Category: Other Cell and Tissue Culture Reagents Tags: Cell Culture, Essential Amino Acids, MEM, Non-Essential Amino Acids

MEM Essential & Non-Essential Amino Acids Solution (50X)

MEM Essential & Non-Essential Amino Acids Solution (50X) are indeed playing a substantial role in cell culture. Essential amino acids cannot be synthesized in vivo,  thus it is extremely important to supplement the media with. On the other hands, mammalian cells can synthesize non-essential amino acids (NEAA) through glutaminolysis, glycolysis, or via TCA cycle. PurMa Biologics Manufactures both MEM Essential & Non-Essential Amino Acids Solution (50X)

SKU Select options below to see it
SKU: N/A Category: Other Cell and Tissue Culture Reagents Tags: Cell Culture, Essential Amino Acids, MEM, Non-Essential Amino Acids

Description

Background

MEM Essential & Non-Essential Amino Acids Solution (50X) is indeed playing a substantial role in cell culture. Basically, Essential amino acids cannot be synthesized In Vivo,  thus it is extremely important to supplement the media with those to maintain proper cellular growth and expected viability. On the other hand, mammalian cells can synthesize non-essential amino acids (NEAA) through glutaminolysis, glycolysis, or via TCA cycle.

During low concentration of required amino acids in media, cells need to consume more glucose and glutamine, which results in an unexpected amount of biodegradation products, this , indeed acts as a hindrance in proper cellular growth and maintenance. So, adding supplements of amino acids to media reduces biosynthetic burden on cell in vitro, stimulates growth and prolongs the viability of cells.

Moreover, you can find the formulation for PurMa™ MEM Essential Amino Acids: Formulation

additionally, you can find the formulation for PurMa™ MEM Non-Essential Amino Acids:Formulation

PurMa Biologics manufactures the essential and non-essential amino acids using our highest quality water, PurMaTM Ultra-Pure Cell Culture Suited water (Cat#: P3W010101). For more information, click here: PurMa™ Ultra-Pure Cell Culture Suited water

Special Features

  • Ready-To-Use.
  • Cell Culture Suited.
  • Sterilized Using 0.2 µm Filters.
  • Additionally,  they are , Vigorously tested for Mycoplasma and Endotoxin
  • and Lastly, all our supplements are Packed Under N2.

References

  1. Dietary requirements of “nutritionally non-essential amino acids” by animals and humans. Wu et al. Amino Acids. 2013 Apr;44(4):1107-13. Epub 2012 Dec 18.
  2. Ingested non-essential amino acids recruit brain orexin cells to suppress eating in mice. Viskaitis et al. Curr Biol. 2022 Apr 25;32(8):1812-1821.e4. Epub 2022 Mar 21.
General specifications
Parameter Specification
Grade Cell Culture Suited
Appearance Clear
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Filtration Sterile filtered using 0.2 µm filter
Indicator NA
Conductivity of Used Water 0.056 µs/cm2
Resistancy of Used Water 18.2 Ωm
Shipping Condition Dry Ice
Refractive index n20/D 1.34 (lit.)
Molecular Biology Parameters
DNases & RNases NOT Detected
proteases NOT Detected
phosphatases NOT Detected
Pathogens Evaluation
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Mold & Fungi/Yeast Negative
Bacteria Negative
Sodium pyruvate NA

 

Additional information

Product

PurMa™ MEM Essential Amino Acids Solution (50X), PurMa™ MEM Non-Essential Amino Acids Solution (50X)
Size

1 x 10 ml, 1 x 100 ml

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

★★★★★
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Gregory Mullen OU Health Sciences Center
★★★★★
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Haus Bio Verified customer
★★★★★
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Verified Customer
★★★★★
Very fast, efficient, and economic service. Great for new PIs like me.
Surendra Shukla OU Health Sciences Center
★★★★★
it is reliable vendor!
Jianhua Song Applied StemCell
★★★★★
We always buy our agarose from PurMa and have never been disappointed.
Zhenghui Liu Applied StemCell

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Frequently asked questions

What concentration is MEM Essential & Non-Essential Amino Acids Solution (50X) supplied at?

Most supplements and solutions are supplied as concentrated stocks, commonly 100X, for dilution into complete medium. The exact concentration is on the Certificate of Analysis for the lot.

How should it be stored?

Follow the storage line on the Certificate of Analysis, and aliquot anything supplied frozen so you are not repeatedly freezing and thawing the stock.

Is it sterility and endotoxin tested?

Yes. Each lot is tested and a Certificate of Analysis is issued and available on request for any catalogue number.

Can I get this in a custom concentration or pack size?

Yes. Custom concentrations, formulations and bulk pack sizes are made to order. Use the request bulk pricing button on this page and state the specification you need.

What is the shelf life?

Stated per lot on the Certificate of Analysis. Once opened the practical limit is usually contamination rather than chemical stability, so handle in a hood and decant rather than pipetting repeatedly from the stock.

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