Description

PurMa™ Mycoplasma Growth Media

PurMa™ Mycoplasma Growth Media is a proprietary combination which provides an excellent environment for various species of mycoplasma to grow.

Nutrition

Mycoplasma has a complex nutritional requirement. This therefore, makes them dependent on external supplies. PurMa™ Mycoplasma Growth Media is ideal for growing different strains. Mycoplasmas have three important features: they are categorized as Mollicutes class, they also possess low GC content, and they basically don’t have cell wall. Additionally, mycoplasma has a simplified metabolic pathway and therefore it is a hard to grow this organism, especially under laboratory conditions.

Mycoplasma Detection

PurMa™ Mycoplasma Growth Media is also used for detecting the exact strain of contaminating mycoplasma.

The best tool for evaluating  the presence of mycoplasma in any liquid such as media is by using the PCR method. The mycoplasma agar bcan also be used by growing mycoplasma and counting mycoplasma fried egg” shape colonies.

• Initially,  Incubate the contaminated samples of media, FBS or cell line in 5 ml of PurMa™ MycoGrowth media (liquid).  The condition must be anaerobic while shaking.
• Pipet 100 µl of the media on an PurMa™ MycoAgar plate.
• After three days individual fried egg structure will start to show up.
• Pick up several colonies and grow them in PurMa™ MycoGrowth liquid media.
• Use PurMa™ Mycoplasma PCR Detection kit (Cat#: P5M011404) to amplify the fragment.
• Have the PCR reaction cleaned and sent for sequencing.
• The 230bp PCR fragment resulted from our kit will clearly determine the strain of your contaminant.

Special Features

• Easy -to- Use
• Is an excellent tool to determine your exact mycoplasma contamination.
• Contains a proprietary formulation which maximizes the growth of all known mycoplasmas.
• Rigorously tested for bacteria, endotoxin, and mold.
• Free of all mycoplasmas inhibiting growth elements.

References

1. DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. TOURTELLOTTE et al. J Bacteriol. 1964 Jul;88(1):11-5.
2. Comparison of undefined medium and its dialyzable fraction for growth of Mycoplasma. Pollock et al. 1969 Feb;97(2):522-5.