Mycoplasma Growth Media

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Mycoplasma Growth Media

Mycoplasma Growth Media

$81.69 – $319.36

PurMa™ Mycoplasma Growth Media is a proprietary combination. This media does not only provide an excellent environment for various species of mycoplasma to grow, but is also used for detecting the exact strain of contaminating mycoplasma.

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Description

PurMa™ Mycoplasma Growth Media

PurMa™ Mycoplasma Growth Media is a proprietary combination which provides an excellent environment for various species of mycoplasma to grow.

Nutrition

Mycoplasma has a complex nutritional requirement. This therefore, makes them dependent on external supplies. PurMa™ Mycoplasma Growth Media is ideal for growing different strains. Mycoplasmas have three important features: they are categorized as Mollicutes class, they also possess low GC content, and they basically don’t have cell wall. Additionally, mycoplasma has a simplified metabolic pathway and therefore it is a hard to grow this organism, especially under laboratory conditions.

Mycoplasma Detection

PurMa™ Mycoplasma Growth Media is also used for detecting the exact strain of contaminating mycoplasma.

The best tool for evaluating  the presence of mycoplasma in any liquid such as media is by using the PCR method. The mycoplasma agar bcan also be used by growing mycoplasma and counting mycoplasma fried egg” shape colonies.

  • Initially,  Incubate the contaminated samples of media, FBS or cell line in 5 ml of PurMa™ MycoGrowth media (liquid).  The condition must be anaerobic while shaking.
  • Pipet 100 µl of the media on an PurMa™ MycoAgar plate.
  • After three days individual fried egg structure will start to show up.
  • Pick up several colonies and grow them in PurMa™ MycoGrowth liquid media.
  • Use PurMa™ Mycoplasma PCR Detection kit (Cat#: P5M011404) to amplify the fragment.
  • Have the PCR reaction cleaned and sent for sequencing.
  • The 230bp PCR fragment resulted from our kit will clearly determine the strain of your contaminant.

Special Features

  • Easy -to- Use
  • Is an excellent tool to determine your exact mycoplasma contamination.
  • Contains a proprietary formulation which maximizes the growth of all known mycoplasmas.
  • Rigorously tested for bacteria, endotoxin, and mold.
  • Free of all mycoplasmas inhibiting growth elements.

References

  1. DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. TOURTELLOTTE et al. J Bacteriol. 1964 Jul;88(1):11-5.
  2. Comparison of undefined medium and its dialyzable fraction for growth of Mycoplasma. Pollock et al. 1969 Feb;97(2):522-5.
Parameter Specification
Appearance Liquid/Powder
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Indicator NA
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid/ powder
Shipping Condition Ambient

Quality Control

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SDS

PurMa™ Mycoplasma Growth Media_SDS

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    Penicillin/Streptomycin/ Neomycin Antibiotic Mix (100X)

    Background

    Penicillin/Streptomycin/ Neomycin Antibiotic Mix (100X) prevents bacterial, mold & virus, mycoplasma, as well as yeast contamination. Moreover, most of the cell lines, unless they are maintained fully aseptic and come from an aseptic facility, need a mixture of antibiotics to prevent various above-mentioned contamination. Furthermore, this antibiotic mix contains all the necessary ingredients that can successfully prevent common contamination.

    Ingredients

    Penicillin 5000 UI/ml
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    Neomycin 10 mg/ml

    Special Features

    • Cost-effective.
    • Effective against both gram-positive and gram-negative bacteria.
    • Contains antibiotics and antimycotics to prevent mycoplasma, fungi, and yeast.
    • Provides harmless an and  healthy environment for cell line if you follow the recommended concentration.
    • More importantly, it does not cause  toxicity level for all the available cell lines.
    • Lastly, we highly recommend performing a dose-response test at the beginning of the study. That will definitely help  deciding the level at which the antibiotic mix will prevent the contamination for each cell line.

    Formulation

    Please find the formulation here :Formulation

    References

    1. Screening of drugs for rapid activity against Trypanosoma cruzi trypomastigotes in vitro. Croft SL, Walker JJ, Gutteridge WE.Trop Med Parasitol. 1988 Jun;39(2):145-8.PMID:
    2. In vitro and in vivo studies of streptomycin-resistant, penicillin-susceptible streptococci from patients with infective endocarditis. Enzler MJ, Rouse MS, Henry NK, Geraci JE, Wilson WR. J Infect Dis. 1987 May;155(5):954-8. doi: 10.1093/infdis/155.5.954. PMID: 364485.
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    Background

    Generally, four types of pathogens contaminate most of the cell lines. Those, however,  include Bacteria, mold/ yeast, virus as well as mycoplasma. Moreover, when cells divides in media , it carries the contamination along to the daughter cells.  Additionally, PurMaTM Penicillin / Streptomycin Antibiotic Mix Solution (100X) contains all the necessary ingredients that can successfully prevent common contamination.

    Ingredients

    Streptomycin 10 mg/mL
    Penicillin 10000 UI/mL

    Specific Features

    • Cost effective!Effectively,.
    • Additionally, removes gram-positive and gram-negative bacteria.
    • Contains antibiotics and antimycotics to prevent mycoplasma and yeast.
    • Does not harm healthy cell lines if it is used in the recommended concentration.
    • More importantly, does not cause toxicity for all the available cell lines.
    • Highly recommend performing a dose-response test at the beginning to decide the level at which determines the level in which the contamination is prevented and at the same time cultured cell and tissues are in their high-performance

    Formulation

    For detailed formulation click here:    Formulation

    References

    1. Antibacterial action of penicillin, penicillin X, and streptomycin on Hemophilus influenzae. HEWITT WL, PITTMAN M. Public Health Rep (1896). 1946 May 31;61:768-78. PMID: 20983031.
    2. The effect of streptomycin and penicillin on the growth and differentiation in vitro of Hymenolepis microstoma. Evans WS. Can J Zool. 1978 May;56(5):1210-1. doi: 10.1139/z78-166. PMID: 667758.
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    Appearance clear Liquid
    Strength 100X
    Reagent types Anti-mycotic, antibiotics
    Form Suspension
    Application For prevention of cell culture contamination
    Culture suitability Mammalian Cell Culture
    Endotoxin  < 0.5 EU/ml
    Sterile Filtered Yes, 0.2µm
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    Mycoplasma PCR Detection Kit

    Background

    Undoubtedly, PurMa Biologics is the pioneering company in both mycoplasma detection and elimination reagents. Moreover, PurMa™ Mycoplasma PCR Detection Kit is fast, easy to use, highly accurate kit. The kit contains a 2X green master mix and needs just 1-2µl of your sample to be tested.

    PurMa™ Mycoplasma PCR Detection Kit (50 Reactions) Cat # P5M011404-50 Contents

    • PurMaTM PCR 2X Mix (containing green dye, no need for loading dye  includes all the primers) (700 µl)
    • It also contains reaction buffer (2500µl)
    • More importantly, Positive control. The lysed mycoplasma which does not carry any live mycoplasma (50ul)
    • Negative Control. Lysed COS1 cells serves as the negative control (mycoplasma free), ready to proceed to the PCR and (50µl).
    • Lastly, PurMa™ Ultra-Pure Nuclease Free Water (Cat# P3W013104) (1ml)
    To gain the best results, please strictly follow the guidance: Protocol

    Mycoplasma Contamination

    Contamination with mycoplasma does not cause turbidity or obvious changes in cell culture media, therefore is under-looked in many laboratories. Although, to date, more than 190 mycoplasma species have been reported, it is eight species that account for more than 95% of all mycoplasma contamination in cell culture, including: M. arginini (bovine), M. fermentans (human), M. hominis (human), M. hyorhinis (porcine), M. orale (human), M. pirum (human), M. salivarium (human), and Acholeplasma laidlawii (bovine). Our sophisticated PurMa™ Mycoplasma PCR Detection Kit not only detects the mycoplasma in your cell culture system, but also determines the exact mycoplasma strain is the cause of contamination.

    Consequences of contamination with mycoplasma

    1. Gradual deterioration of the cell line, which is especially significant in stable cell lines (15-35%)
    2. Inconsistency among the results and much higher standard deviations in your statistical analysis.
    3. Altering DNA, RNA, and protein synthesis.
    4. Diminishing amino acid and ATP levels.
    5. Chromosomal alterations.
    6. Modifying host-cell immune system.

    Mycoplasma contamination can happen in various ways

    1. Faulty laboratory equipment. (For Instance: a dysfunctioning laminar flow could let mycoplasma-containing dust in the biosafety cabinet, thereby causing contamination of all of the media and cells within.)
    2. Inadequate culturing techniques, human breath contains several major mycoplasma including M. fermentans which count for the majority of cell culture contamination (45%). %). Wearing a mask is absolutely needed while working in a cell culture lab, especially when checking cells under microscope.
    3. Getting a cell line from another laboratory may introduce Mycoplasma contamination.

    Mycoplasma can affect the phenotypic and functional characteristics of cells In Vitro

    Including:
    • Chromosomal aberrations
    • Disruption of nucleic acid synthesis
    • Changes in membrane antigenicity
    • Inhibition of cell proliferation and metabolism
    • Decreased transfection rates
    • Changes in gene expression profiles
    • Affects virus production.
    • Cell death

    Special Features

    • PurMaTM Mycoplasma PCR Detection Kit is Easy to Use
    • The procedure is fast and is completed within 2 hrs.
    • Our kit has high detection ability.
    • Does not detect bacterial or animal sequences of DNA.
    • PurMaTM Mycoplasma PCR Detection Kit detects the following species:  A. Laidlawii, M. Arginine, M. Boris, M. Fermentans M. Hominis, M. Hyorhinis and M. Orale
    • PurMaTM Mycoplasma Detection Kit detects a 230 bp amplicon.
    • The kit contains a 2X ready master mix containing all the primers and green dye (you would not need to add loading dye after amplification to tun the result on agarose gel) and needs only 1-2µl of your sample to be tested.

    References

    1. Mycoplasma detection in cell culture by concomitant use of bisbenzamide and fluoresceinated antibody. Freiberg et al. In Vitro Cell Dev Biol. 1990 Jun;26(6):585-8.
    2. Mycoplasma detection by PCR analysis. Hopert et al. In Vitro Cell Dev Biol Anim. 1993 Oct;29A(10):819-21.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="12" fusion_table_columns="2" margin_top="" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Clear liquid/Green
    pH 7.4 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Indicator NA
    Sterility Tested Sterile filtered using 0.1 µm filter
    Form Liquid
    Shipping Condition Dry Ice
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container] Read more

  • Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X)

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    Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X)

    Background

    PurMaTM Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X) generally prevents contamination of the four main pathogens in cell culture. Those are bacteria,  mold/ yeast, virus as well as mycoplasma. Moreover, when cells divide in media, it carries contamination along to the daughter cells.  Additionally, low fungicide antibiotic mix suites cells show sensitivity to higher amount of Amphotericin B.

    Ingredients

    Streptomycin 10 mg/mL
    Penicillin 10,000 UI/mL
    Amphotericin B 2.5 µg/mL
     

    Special Features

    • Cost-effective.
    • Effective against both gram-positive and gram-negative bacteria.
    • Contains antibiotics and antimycotics to prevent mycoplasma, fungi, and yeast.
    • Additionally. designed to be harmless to a healthy cell line if it is used in the recommended concentration.
    • More importantly, it does not cause toxicity for all the available cell lines.
    • Highly recommend performing a dose-response test at the beginning to decide the level at which determines the level in which the contamination is prevented and at the same time cultured cells and tissues are in their high-performance

    Formulation

    For the formulation, Click here:Formulation

    References

    1. Risk factors for amphotericin B-induced nephrotoxicity. Luber et al. J Antimicrob Chemother. 1999 Feb;43(2):267-71. doi: 10.1093/jac/43.2.267. PMID: 11252333.
    2. Screening of drugs for rapid activity against Trypanosoma cruzi trypomastigotes in vitro. Croft et al.Trop Med Parasitol. 1988 Jun;39(2):145-8.
    [/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset="" /][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml Read more

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