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PurMaFectin™ Ultra Transfection Reagent

PurMaFectin™ Ultra Transfection Reagent

(18 customer reviews)

PurMa Biologics manufactures PurMaFectin™ Ultra Transfection Reagent wth over 97% efficacy. Also, this reagent performs as non-liposomal. Our Ultra Transfection Reagent provides consistent/reproducible transfection with ignorable cytotoxicity PurMaFectin™ Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian/ eukaryotic nucleus.

SKU: N/A Category: Cell Culture Suited Antibiotics Tags: Antibiotic Mix Solution, Bacteria, Mold, Mycoplasma, Penicillin, Streptomycin, Virus, Yeast

Description

Background

PurMa Biologics manufactures PurMaFectin™ Ultra Transfection Reagent wth over 97% efficacy. Also, this reagent performs in non-liposomal mmanner. Our Ultra Transfection Reagent provides consistent/reproducible transfection with ignorable cytotoxicity PurMaFectin™ Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian/ eukaryotic nucleus.

Special Features

For complete protocol, click here: PurMaFectin™ Ultra_ Use Manual

    • Basically, the entire procedure with PurMaFectin™ Ultra is completed within 30 minutes.
    • Furthermore, for the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. We strongly recommend starting the seeding by counting the trypsinized cells so there will be high consistency among your experiments.
    • Additionally, different cell types react differently to the number of passages when they are used for transfection. So, to minimize the impact of passage on efficiency of transfection, either use the cells with the same passage number. Alternatively, apply at least two different concentrations of transfection reagent as control in new transfect experiments before the condition is optimized.
    • We deplete endotoxin from our transfection reagent. The  endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. To deplete the endotoxin, we use PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). In this proprietary kit, Sepharose 6B covalently conjugates to  an endotoxin-specific absorbent. The mentioned procedure will end with passing the reagent through 0.1µm filters 3X (included in the kit). The mentioned  eliminates the residual endotoxin.
Parameter Specification
Appearance clear liquid, Red liquid
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator NA
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition Dry Ice

References

  1. Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents. Rahimi et al. 2018;119(11):701-705. doi: 10.4149/BLL_2018_125. PMID: 30686003.
  2. Transfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells. Zhang et al. Biotechniques. 1996 Nov;21(5):940-2, 944-5. doi: 10.2144/96215pf02. PMID: 8922638.

 

Additional information

Size

1 x 1 ml, 1 x 10 ml, 1 x 100 ml

18 reviews for PurMaFectin™ Ultra Transfection Reagent

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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