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Serum Free Cryoprotective Media (SFCM) DMSO Free
PurMa™ Serum Free Cryoprotective Media (SFCM); DMSO Free is free of animal products and is fully synthetic. Serum and its components are well-known for their beneficial effect on cells during cryopreservation. Nevertheless, there is known disadvantages of the serum in Cryoprotective media.
Description
Background
PurMa Biologics has spent several decades to find a replacement for serum in cryoprotective media. We have evaluated different combinations of synthetic polymers that can replace serum and have successfully developed a fully defined cryoprotective media. PurMaTM Serum Free Cryoprotective Media (SFCM); DMSO Free is free of animal products and is fully synthetic.
Disadvantages of the Serum in Cryoprotective Media
Serum and its components are well-known for their beneficial effect on cells during cryopreservation. Nevertheless, there are known disadvantages of serum in cryoprotective media:
- The inability to fully sterilize them.
- They contain an unknown composition.
- The variation from batch to batch. This results in inconsistency in the rate of survival, recovery, and viability in cryopreservation.
Therefore, a chemically defined Cryoprotective media resolves the several restrictions including the above-mentioned issues.
Although DMSO, with its penetrating ability, is a strong cryoprotective agent, there are, however, some limitations and disadvantages. For more details about the beneficial role of DMSO, please see our other cryoprotective media with DMSO, such as:
limitations and disadvantages of DMSO
- Basically, it has a broad toxicity.
- Additionally, it modifies the expression of cells such an NK and T cells
- It alters the mRNA level of the de novo DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.
- Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.
- It damages the mitochondria in certain cell culture models such as astrocytes.
- It negatively impacts cellular membrane/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.
- Besides, it induces unwanted stem cell differentiation.
- Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.
- Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.
PurMa™ Serum Free Cryoprotective Media DMSO Free (SFCM) prevents cell dehydration and formation of ice crystals. So, it makes an excellent choice for short- and long-term cryopreservation. Moreover, PurMaTM SFCM allows this media to preserve your sensitive cells without penetrating the cells.
Please follow the exact protocol as using non penetrating cryomedia requires slow freezing (1°C/ min)
Special Features
- Ready-to-Use medium.
- It is free of serum and DMSO ( or any other penetrating cryoprotective agents)
- Each and every Lot of PurMaTM Serum Free Cryoprotective Medium (SFCM), besides the pathogens and sterility evaluations, goes through a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and testing for the rate of recovery by Alamar Blue test.
- PurMaTM Serum Free Cryoprotective Medium (SFCM) has been designed for the lowest cell loss during freezing and thawing & highest cell recovery and is proven to have over 62% survival and recovery rate after long term cryopreservation.
- Sterile (Passed through 0.2µm filters).
- PurMaTM Serum Free Cryoprotective Media (SFCM); DMSO Free has proven successful for primary and commercial cell lines.
- Suitable for clinical applications.
Guideline
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines
References
- Cryopreservation of stromal vascular fraction of adipose tissue in a serum-free freezing medium. Thirumala et al. J Tissue Eng Regen Med. 2010 Mar;4(3):224-32. doi: 10.1002/term.232. PMID: 19967746.
- Phase transitions and mechanisms of cryoprotection of serum-/xeno-free media based ondextran and dimethyl sulfoxide. Pakhomov et al. Cryobiology. 2022 Aug;107:13-22. doi: 10.1016/j.cryobiol.2022.06.004. Epub 2022 Jun 24.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is Cryoprotective Media?
Additional information
| Size | 1 x 10 ml, 1 x 100 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Frequently asked questions
Should I choose the DMSO or DMSO free formulation?
DMSO is the standard cryoprotectant and gives the best recovery for most lines. Choose DMSO free where DMSO would interfere with the application, for example in differentiation work or certain clinical and hybridoma protocols where residual DMSO is a problem.
What freezing rate should I use?
Approximately minus 1 degree C per minute to around minus 80, using a controlled rate cooler or an insulated freezing container, before transfer to liquid nitrogen. Faster cooling causes intracellular ice and slower cooling causes osmotic damage.
How should vials be stored long term?
In the vapour phase of liquid nitrogen, below minus 150 degrees C. Storage in a minus 80 freezer is acceptable only for weeks, not years, because viability declines steadily above minus 130.
How should I thaw cells frozen in Serum Free Cryoprotective Media (SFCM) DMSO Free?
Thaw rapidly in a 37 degree C bath until only a small ice crystal remains, then dilute promptly into pre warmed medium. DMSO is toxic to cells at room temperature, so the interval between thaw and dilution should be as short as possible.
How should the cryoprotective media itself be stored?
Store as stated on the Certificate of Analysis, protected from light. Do not refreeze after thawing the media, and use within the period stated for the lot.
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