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- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
PurMaFectin™ Ultra Transfection Reagent
PurMa Biologics manufactures PurMaFectin™ Ultra Transfection Reagent wth over 97% efficacy. Also, this reagent performs as non-liposomal. Our Ultra Transfection Reagent provides consistent/reproducible transfection with ignorable cytotoxicity PurMaFectin™ Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian/ eukaryotic nucleus.
Description
Background
PurMa Biologics manufactures PurMaFectin™ Ultra Transfection Reagent with over 97% efficacy. Also, this reagent performs in a non-liposomal manner. Our Ultra Transfection Reagent provides consistent/reproducible transfection with ignorable cytotoxicity. PurMaFectin™ Ultra combines with DNA, protects it from lysosomal degradation and facilitates efficient plasmid delivery to mammalian/ eukaryotic nucleus.
Special Features
For complete protocol, click here: PurMaFectin™ Ultra_ Use Manual
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- Basically, the entire procedure with PurMaFectin™ Ultra is completed within 30 minutes.
- Furthermore, for the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. We strongly recommend starting the seeding by counting the trypsinized cells so there will be high consistency among your experiments.
- Additionally, different cell types react differently to the number of passages when they are used for transfection. So, to minimize the impact of passage on efficiency of transfection, use the cells with the same passage number, or alternatively, apply at least two different concentrations of transfection reagent as control in new transfect experiments before the condition is optimized.
- We deplete endotoxin from our transfection reagent. The endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. To deplete the endotoxin, we use PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). In this proprietary kit, Sepharose 6B covalently conjugates to an endotoxin-specific absorbent. The mentioned procedure will end with passing the reagent through 0.1µm filters 3X (included in the kit). The mentioned procedure eliminates the residual endotoxin.
| Parameter | Specification |
| Appearance | clear liquid, Red liquid |
| pH | 7.4 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | NA |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Dry Ice |
References
- Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents. Rahimi et al. 2018;119(11):701-705. doi: 10.4149/BLL_2018_125. PMID: 30686003.
- Transfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells. Zhang et al. Biotechniques. 1996 Nov;21(5):940-2, 944-5. doi: 10.2144/96215pf02. PMID: 8922638.
Additional information
| Size | 1 x 1 ml, 1 x 10 ml, 1 x 100 ml |
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Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
Frequently asked questions
Should serum be present during transfection?
It depends on the reagent. Many lipid-based reagents form complexes better in serum-free medium, with serum added back after a few hours. Check the protocol for the specific product rather than assuming.
How do I optimise the reagent to DNA ratio?
Run a small matrix on your specific cell line, varying reagent volume against a fixed DNA amount. The optimum differs markedly between lines and is the single biggest determinant of efficiency.
Why is my transfection efficiency low?
The usual causes are cell confluence outside the optimal window, poor quality or endotoxin-contaminated DNA, too little or too much reagent, and antibiotics in the medium during transfection increasing toxicity.
Is the reagent toxic to cells?
All transfection reagents carry some toxicity, which rises with concentration and exposure time. If viability drops sharply, reduce the reagent amount and shorten the exposure before assuming the cells are the problem.
How should PurMaFectin™ Ultra Transfection Reagent be stored?
At the temperature stated on the Certificate of Analysis, usually 2 to 8 degrees C, and not frozen unless the label says so. Freezing many lipid formulations destroys them irreversibly.
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