Dulbecco’s Modified Eagle’s Medium (DMEM)
Dulbecco’s Modified Eagle’s Medium is a modification of Basal Medium Eagle. Our DMEM, optimized to deliver exceptional growth and consistency, is available in Standard, MAX, and Advanced formulations. Furthermore, PurMa Biologics currently manufactures 128 conditions of this media.
Description
Background:
Dulbecco’s Modified Eagle’s Medium (DMEM)
is a modification of Basal Medium Eagle (BME). Currently, DMEM is one of the most widely used media.
Major modifications compared to the original formulation:
- Although the original Dulbecco’s Modified Eagle’s Medium contained 1000 mg/L of glucose, there are different Glucose amounts used. As shown below, PurMa Biologics DMEM contains 4.5 g/L glucose. Additionally, PurMa Biologics offers a (1.5 g/L) low glucose (P3SO28105) condition.
- Furthermore, DMEM contains fourfold more amino acids and vitamins compared to the original formulation. DMEM is a good choice for growing the primary cell culture of mouse and chicken cells. Moreover, for a variety of normal and transformed cells, this media can be used properly. Each of these DMEMs, by comparison, offers a different combination of L-glutamine and sodium pyruvate.
- More importantly, DMEM has several fold more buffering capacities (3.7g/L NaHCO3) Need of FBS.
Lastly, DMEM Contains:
Furthermore, DMEM contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
- High glucose (4.5g/L)
- Non-essential amino acids
- Sodium pyruvate (0.11 g/L)
- Phenol red (0.0015g/L)
- L-glutamine (0.585 g/L)
Types of DMEM
Currently, PurMa Biologics Manufactures three forms of DMEM: Standard, MAX, and Advanced. Moreover, the optimization of these three forms of DMEM is the outcome of decades of cell culture research and experience. Undoubtedly, our DMEM delivers exceptional growth and consistency for your cell culture projects.
Formulation:
Complete formulation is available here: DMEM Formulation
Reference:
- The viability change of pigskin in vitro. Ge L, et al. 2010 Jun;36(4):533-8.
- A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Soleimani M, Nadri S.Nat Protoc. 2009;4(1):102-6.
rile filtered using 0.22 µm filter
Liquid
| Parameter | Specification | |
|---|---|---|
| Appearance | Red, clear liquid | |
| pH | 7.2 ± 0.1 | |
| Osmolality | 275-360 mOsm/L | |
| Endotoxin | NMT< 2EU/mL | |
| Mycoplasma | Negative | |
| Suitability | Suitable for mammalian cell culture | |
| Additive | Sodium pyruvate | |
| Indicator | Phenol red | |
| Mycoplasma Detection | Negative | |
| Sterility Tested | Ste | Form |
| Shipping Condition | Room temperature |
Additional information
| Condition | DMEM- Standard Formulation, DMEM- Standard Formulation, ATCC Formulation, DMEM- Without Glutamine, DMEM- Without Phenol Red, DMEM- Without Sodium Bicarbonate, DMEM- With Sodium Pyruvate, DMEM- Without Glucose, DMEM- With Lipoic Acid, DMEM- Without Glutamine, Without Phenol Red, DMEM- Without Glutamine, Without Sodium Bicarbonate, DMEM- Without Glutamine, Without Phenol Red, Without Sodium Bicarbonate, DMEM- Low Glucose (1.5 g/L), DMEM- Low Sodium Bicarbonate (1.5 g/L), DMEM- 15 mM HEPES (3.6 g/L), DMEM- 25 mM HEPES (5.9 g/L), DMEM- 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, Without Sodium Bicarbonate, DMEM- Without phenol Red, 15 mM HEPES (3.6 g/L), DMEM- Without phenol Red, 25 mM HEPES (5.9 g/L), DMEM- Without phenol Red, Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
18 reviews for Dulbecco’s Modified Eagle’s Medium (DMEM)
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5
hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
Have you purchased: Yes
Time of submission: 17-Mar-2025 12:09:58
Ease of placing the order: 5/5
jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
Have you purchased: Yes
Time of submission: 17-Mar-2025 14:22:58
Ease of placing the order: 4/5
tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
Have you purchased: Yes
Time of submission: 18-Mar-2025 10:01:50
Ease of placing the order: 5/5
Fitzkr@gmail.com –
Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
Always great quality, fast processing and shipping.
We are totally satisfied.
Have you purchased: Yes
Time of submission: 22-Mar-2025 10:47:00
Ease of placing the order: 5/5
vmadka@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:02:40
Ease of placing the order: 4/5
Anupam-Mandal@ouhsc.edu –
Review: Excellent
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:05:43
Ease of placing the order: 5/5
rohan-varshney@ouhsc.edu –
Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:06:38
Ease of placing the order: 5/5