Advanced Leibovitz’s L-15 Medium
Advanced Leibovitz’s L-15 Medium contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Furthermore, Advanced Leibovitz’s L-15 needs 2-5% FBS and additionally provides more consistency.
Description
Advanced Leibovitz’s L-15 Medium
Background
PurMaTM Advanced Leibovitz’s L-15 Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Leibovitz’s L-15, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Leibovitz’s L-15 Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Leibovitz’s L-15 Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Leibovitz’s L-15 (CD-A-Leibovitz’s L-15) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced Leibovitz’s L-15 Formulation
References
- The effect of a phorbol ester on amphibian myogenesis. Zeng MB, et al. 1990 Mar;23(1):106-15. PMID: 2382522.
- Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish. Ishida AT, Cheng MH.Exp Eye Res. 1991 Feb;52(2):175-91. doi: 10.1016/0014-4835(91)90257-f.PMID: 2013300
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A- Leibovitz’s L-15 Standard Formulation, A- Leibovitz’s L-15 w/o L-Alanyl-L-Glutamine, A- Leibovitz’s L-15 w/o Phenol Red, A- Leibovitz’s L-15 w/o Sodium Pyruvate, A- Leibovitz’s L-15 w/o Galactose, A- Leibovitz’s L-15 High Galactose (4.5 g/L), A- Leibovitz’s L-15, 15 mM HEPES (3.6 g/L), A- Leibovitz’s L-15, 25 mM HEPES (5.9 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]SDS
SDS
Only logged in customers who have purchased this product may leave a review.
Related products
-
- Sale!
- Dulbecco’s Modified Eagle’s Medium (DMEM), Cell Culture Media
Dulbecco’s Modified Eagle’s Medium (DMEM)
- $21.59 – $518.27
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Eagle's Minimal Essential Medium (EMEM), Cell Culture Media
Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)
- $29.15 – $547.30
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Roswell-Park Memorial Institute 1640, Cell Culture Media
Roswell Park Memorial Institute 1640 (RPMI-1640)
- $21.70 – $549.37
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews
There are no reviews yet.