Advanced McCoy’s 5A Modified Media (A-M5AMM)
Advanced McCoy’s 5A Modified Media (A-M5AMM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced McCoy’s 5A needs 2-5% FBS and provides more consistency.
Description
Advanced McCoy’s 5A Modified Media (A-M5AMM)
Background
PurMa™ Advanced McCoy’s 5A Modified Media (A-M5AMM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In Advanced McCoy’s 5A, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced McCoy’s 5A Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- Advanced McCoy’s 5A incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMa™ Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced McCoy’s 5A Modified Media (A-M5AMM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced McCoy’s 5A (CD-A-M5AMM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced McCoy’s 5A Formulation
References
- Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-M5AMM- Standard Formulation, A-M5AMM Without o L-Alanyl-L-Glutamine, A-M5AMM -Without Phenol Red, A-M5AMM -Without Sodium Bicarbonate, A-M5AMM – Without Glucose, A-M5AMM – Without Peptone, A-M5AMM – w/o o L-Alanyl-L-Glutamine, w/o Phenol Red, A-M5AMM – Without Phenol Red, Without Sodium Bicarbonate, A-M5AMM – w/ o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-M5AMM – Low Glucose (1.5 g/L), A-M5AMM – Low Sodium Bicarbonate (0.35 g/L), A-M5AMM – Low Sodium Bicarbonate (0.85 g/L), A-M5AMM -15 mM HEPES (3.6 g/L), A-M5AMM – 25 mM HEPES (5.9 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
SDS
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