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PurMa™Chemically Defined (CD) (CD-DMEM)

PurMa™Chemically Defined (CD) (CD-DMEM)

(43 customer reviews)

PurMa Biologics introduces a next-generation DMEM-based medium that is fully chemically defined, serum-free, and free of any animal-derived components. PurMa™Chemically Defined (CD) (CD-DMEM) designed for demanding research and bioprocessing applications, this advanced formulation supports robust cell growth, viability, and productivity while eliminating lot-to-lot variability associated with FBS. Optimized for a wide range of mammalian cell lines, it provides consistent performance in both small-scale experiments and scalable production workflows. With a transparent of PurMa™Chemically Defined (CD) (CD-DMEM), fully disclosed formulation and strict manufacturing controls, this medium delivers the reliability required for reproducible data, regulatory alignment, and streamlined technology transfer from R&D to GMP environments.

SKU: N/A Categories: Cell Culture Media, Dulbecco’s Modified Eagle’s Medium (DMEM) Tags: Advanced, Cell Culture, DMEM, Dulbecco's, Human Transferrin, L-Alanyl-L-Glutamine, Recombinant Insulin, Serum Reduction

Description

PurMa™Chemically Defined (CD) (CD-DMEM)

Background

Modern cell culture demands a medium that delivers consistent performance, regulatory confidence, and freedom from undefined animal components. CD-DMEM is just an examples of chemically defined media manufactured by PurMa Biologics. We are proud to have been able to manufacture highest quality of other form of conventional media:  CD-DMEM/F12,. CD-RPMI-1640, CD- HamF12-K as chemically defined media examples

PurMa Biologics has developed an  advanced next generation  chemically chemically defined media with the DMEM base:  PurMa™Chemically Defined (CD) (C-DMEM) which, serum-free, and free of any trace of animal-derived materials, including FBS and other complex supplements. PurMa™Chemically Defined (CD) (C-DMEM) is engineered to provide a reliable, high-performance platform for both research and bioprocessing, supporting robust growth and productivity across a broad range of mammalian cell types.

Traditional DMEM formulations often rely on fetal bovine serum to supply growth factors, hormones, and binding proteins. While effective in many laboratory settings, serum introduces significant sources of variability, batch-to-batch inconsistency, and regulatory challenges. By eliminating FBS entirely and replacing it with precisely defined components, this formulation delivers a highly controlled environment in which every ingredient is known, quantified, and consistent from lot to lot.

PurMa™Chemically Defined (CD) (CD-DMEM) is carefully balanced with amino acids, vitamins, trace elements, inorganic salts, and energy sources that are optimized to support cell proliferation, viability, and metabolic stability. Growth factors and other key components are provided in recombinant or synthetic forms, ensuring that no animal- or human-derived materials enter the workflow. This design makes the product particularly suitable for projects that require xeno-free conditions, downstream clinical translation, or alignment with evolving regulatory expectations for biologics manufacturing.

One of the core advantages of PurMa™Chemically Defined (CD) (CD-DMEM)  is improved reproducibility. By removing the unpredictable variability associated with serum, researchers and process developers can more confidently compare data between experiments, laboratories, and production runs. This consistency is especially important in applications such as monoclonal antibody production, recombinant protein expression, viral vector manufacturing, and stable cell line development, where small changes in medium composition can influence yield and product quality.

PurMa Biologics manufactures PurMa™Chemically Defined (CD) (CD-DMEM) under stringent quality systems, with tight specifications for raw materials, in-process controls, and final product release testing. Each lot is produced with the same rigor, enabling a smooth transition from discovery studies to pilot scale and, ultimately, to large-scale production. The transparent formulation also simplifies technology transfer and documentation, reducing the need for extensive requalification studies when moving between sites or contract manufacturing partners.

In practical use, PurMa™Chemically Defined (CD) (CD-DMEM) is designed for ease of integration into existing workflows. It can be used as a direct replacement for serum-containing DMEM in many adherent or suspension cultures, often with minimal adaptation steps. Users benefit from cleaner downstream processing, reduced risk of adventitious agents, and simplified regulatory filing due to the absence of undefined components and animal-derived supplements.

PurMa Biologics supports this product with technical documentation, application guidance, and scientific expertise to help laboratories optimize protocols for their specific cell lines and target molecules. Whether used in early-stage research, process development, or preclinical manufacturing, this medium provides a robust, future-ready foundation for high-performance, serum-free, and animal-component–free cell culture.

Special Features

– PurMa™Chemically Defined (CD) (CD-DMEM) Completely serum-free and free of any animal-derived components, including FBS

-PurMa™Chemically Defined (CD) (CD-DMEM is an excellent choice for stem cell cultures

– Moreover, Fully chemically defined formulation with transparent, disclosed composition

– Optimized nutrient balance to support high cell viability and sustained proliferation

– PurMa™Chemically Defined (CD) (CD-DMEM) Suitable for both adherent and suspension mammalian cell cultures

– PurMa™Chemically Defined (CD) (CD-DMEM) Xeno-free design that supports translational and preclinical applications

– Tight lot-to-lot consistency for improved reproducibility and data reliability

– Compatible with monoclonal antibody, recombinant protein, and viral vector production

– Manufactured under rigorous quality systems with controlled raw materials

– PurMa™Chemically Defined (CD) (CD-DMEM) facilitates smoother technology transfer from R&D to large-scale manufacturing

– Reduced risk of adventitious agents and simplified regulatory documentation

 

FAQ

Q1. What is a chemically defined (CD) media?
A1. A CD medium  is a cell culture formulation in which every component and its concentration are precisely known. It contains only pure, identified substances such as amino acids, vitamins, salts, and defined growth factors, without any complex, undefined mixtures like serum or tissue extracts. This allows for greater consistency, reproducibility, and easier optimization of culture conditions.

Q2. What makes this DMEM-based medium fully chemically defined?
A2. Every ingredient in the formulation is individually specified and quantified, and all functional components are provided in synthetic or recombinant forms. There are no serum additives or hydrolysates, so the composition is completely transparent and consistent from lot to lot.

Q3. Does this medium contain any fetal bovine serum or other animal-derived components?
A3. No. The formulation is entirely serum-free and does not include fetal bovine serum, animal-derived hydrolysates, or other components of animal origin. This supports xeno-free workflows and reduces regulatory concerns related to animal-derived materials.

Q4. For which cell types is this medium suitable?
A4. It is designed to support a broad range of mammalian cell lines used in research and bioprocessing, such as cells for antibody production, recombinant protein expression, and vector manufacturing. Some specialized or primary cells may require additional optimization or supplements.

Q5. Can this product replace serum-containing DMEM directly?
A5. In many cases it can, with minimal process changes. Certain cell lines adapt quickly, while others benefit from a stepwise transition where serum levels are gradually reduced over several passages to maintain viability and performance.

Q6. Is this medium appropriate for projects with regulatory or clinical goals?
A6. Yes. Its defined composition, absence of animal-derived materials, and controlled manufacturing process make it a strong choice for projects that need robust documentation, reduced variability, and alignment with regulatory expectations in translational and preclinical development.

Formulation:

PurMa™Chemically Defined (CD) (CD-DMEM) Medium includes a base formulation including L-Alanine -L Glutamine : Base Formulation of CD-DMEM_ P3C310105-1

Additionally, PurMa™Chemically Defined (CD) (CD-DMEM)  Medium contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which make it independent of any serum and any trace of animal products.

References:

  1. Development of a CD Medium for in vitro Expansion of Primary Bovine Satellite Cells. Kolkmann AM. et al, 2022, 4;10:895289
  2. CNTF+BDNF treatment and neuroprotective pathways in the rd1 mouse retina. Azadi, S. et al, 2007, 19;1129(1):116-29
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 292-335 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.1 µm,  filter 3times
Form Liquid
Shipping Condition  Room temperature, Overnight

Additional information

Condition

CD-DMEM – Standard Formulation, CD-DMEM – Without L-Alanyl-L-Glutamine, CD-DMEM – Without Phenol Red, CD-DMEM – Without Sodium Bicarbonate, CD-DMEM – Without Glucose, CD-DMEM – Low Glucose (1.5 g/L), CD-DMEM – Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – 15 mM HEPES (3.6 g/L), CD-DMEM – 25 mM HEPES (5.9 g/L), CD-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, CD-DMEM – Without L-Alanyl-L-Glutamine, Without Sodium Bicarbonate, CD-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Bicarbonate, CD-DMEM – 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – Without Phenol Red, Without Sodium Bicarbonate, CD-DMEM – Without phenol Red, 15 mM HEPES (3.6 g/L), CD-DMEM – Without phenol Red, 25 mM HEPES (5.9 g/L), CD-DMEM – Without phenol Red, Low Sodium Bicarbonate (1.5 g/L)
Size

1X 100 ml, 1X 1000 ml, 1X 500 ml

43 reviews for PurMa™Chemically Defined (CD) (CD-DMEM)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

  19. Surendra-Shukla@ouhsc.edu –

    Review: Very fast, efficient, and economic service. Great for new PIs like me.
    Have you purchased: Yes
    Time of submission: 25-Apr-2025 14:00:04
    Ease of placing the order: 5/5

  20. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  21. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  22. anil-singh@ouhsc.edu –

    Review: NA
    Have you purchased: Yes
    Time of submission: 25-Mar-2025 10:11:14
    Ease of placing the order: 5/5

  23. Surendra-Shukla@ouhsc.edu –

    Review: Best quality products, best price.
    Have you purchased: Yes
    Time of submission: 18-Apr-2025 09:51:03
    Ease of placing the order: 5/5

  24. madhusmita-rout@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 21-Apr-2025 11:15:46
    Ease of placing the order: 5/5

  25. alexholloway@mesogen.com –

    Review: This serum appears to be a high quality product and worked well for our needs. Purma has an extensive catalog for cell culturing supplies which we are glad we came across.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Apr-2025 16:54:29
    Ease of placing the order: 5/5

  26. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  27. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  28. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  29. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  30. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  31. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  32. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  33. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  34. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  35. Ravi-Gor@ouhsc.edu –

    Review: I recently had the pleasure of interacting with Dr. Seif Azadi, and I want to express my gratitude for his expertise and timely assistance. His knowledge and dedication were instrumental in addressing our lab’s needs efficiently.
    Have you purchased: It is my first Purchase
    Time of submission: 25-Jun-2025 12:00:09
    Ease of placing the order: 5/5

  36. xiangpeng-li@ou.edu –

    Review: PurMa helped us with the customized medium. They are professional and friendly. I appreciate their great work.
    Have you purchased: Yes
    Time of submission: 14-Aug-2025 12:59:31
    Ease of placing the order: 5/5

  37. jianhua.song@appliedstemcell.com –

    Review: it is reliable vendor!
    Have you purchased: Yes
    Time of submission: 07-Oct-2025 17:14:12
    Ease of placing the order: 5/5

  38. Zhenghui.Liu@appliedstemcell.com –

    Review: We always buy our agarose from PurMa and have never been disappointed.
    Have you purchased: Yes
    Time of submission: 08-Oct-2025 11:17:53
    Ease of placing the order: 5/5

  39. ravi-gor@ou.edu –

    Review: I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency.
    We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments.
    Thank you, Team Purma Biologics, for your good products and support!
    Have you purchased: It is my first Purchase
    Time of submission: 23-Oct-2025 17:30:25
    Ease of placing the order: 5/5

  40. info@takbiotechs.com –

    Review: We are extremely satisfied with the quality and consistency of PURMA Biologics products. Both the FBS and cell culture media have delivered excellent performance in our applications, with reliable batch-to-batch consistency. Highly recommended for research and bioprocessing use.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 14:49:27
    Ease of placing the order: 5/5

  41. aslan.batur@aytakbiotech.com –

    Review: We’ve had a great experience with PURMA Biologics products. In particular, their FBS has shown excellent consistency and performance, and their DMEM media has worked very well in our cell culture applications. Overall, very reliable quality.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 14:57:39
    Ease of placing the order: 5/5

  42. maya.hart@takbiomed.com –

    Review: It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 15:06:47
    Ease of placing the order: 5/5

  43. hajipour@etsu.edu –

    Review: PurMa products are of excellent quality and highly reliable.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 16:23:59
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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