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MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside and, furthermore depending on cell types, laboratories use MEM-α-N for growing mammalian cells.
Description
Background
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. Depending on cell types, laboratories use MEM-α-N for growing mammalian cells for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.
Ingredients
MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) contains:
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
PurMa™ MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) includes Earles’ Buffer
As the composition of Earls buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa™ MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) media also contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
- PurMa Biologics manufactures 121 types of MEM -α-N-E
The nucleoside modifies this media from MEM-α
it contains:
- Ribonucleosides
- Deoxyribonucleosides
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.
Application
This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, PurMa™ MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.
Formulation
Click here for complete formulation as well as in ” Formulation Tab”: MEM alpha with Nucleoside Formulation
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
- Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM-α-N Standard Formulation, MEM-α-N w/o Glutamine, MEM-α-N w/o Phenol Red, MEM-α-N w/o Sodium Bicarbonate, MEM-α-N w/o Glucose, MEM-α-N w/o Lipoic Acid, MEM-α-N w/o Glutamine, w/o Phenol Red, MEM-α-N w/o Glutamine, w/o Sodium Bicarbonate, MEM-α-N w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N 15 mM HEPES (3.6 g/L), MEM-α-N 25 mM HEPES (5.9 g/L), MEM-α-N w/o Sodium Pyruvate, MEM-α-N High Glucose (4.5 g/L), MEM-α-N Low Sodium Bicarbonate (0.35 g/L), MEM-α-N Low Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Frequently asked questions
How should I store MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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