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Penicillin/Streptomycin Antibiotic Mix (100X)
Penicillin/Streptomycin Antibiotic Mix (100X) prevents bacterial, mold & virus as well as yeast and to some extent mycoplasma . Moreover, most of the cell lines, unless they are maintained fully aseptic and come from an aseptic facility, need a mixture of antibiotics to prevent various above-mentioned contamination.
Description
Background
PurMa™ Penicillin/Streptomycin Antibiotic Mix (100X) contains all the necessary ingredients which successfully prevent common contaminants.
In mammalian cell culture, we always need to prevent contamination of four types of contaminants which most of the cell lines need to overcome : Bacterial contamination, mold & virus contamination, mycoplasma contamination, as well as yeast contamination. Commonly, most of the cell lines, unless they are maintained fully aseptic and come from an aseptic facility, need a mixture of antibiotics to prevent above-mentioned contaminants. PurMaTM Penicillin/ Streptomycin Mix contains
Ingredients
| Streptomycin | 10 mg/mL |
| Penicillin | 10000 UI/mL |
Features and benefits:
- Cost effective!
- Effectively removes both gram-positive and gram-negative bacteria.
- Moreover, it contains antibiotics and antimycotics to prevent yeast and mycoplasma partially. PurMa Biologics, however, manufactures PurMa™ Mycoplasma Treatment Kit (MTK) (100X) (Cat:P5M010408) which successfully eliminates mycoplasma from cell culture without harming most of the cell lines.
- Does not harm a healthy cell line if it is used in the recommended concentration.
- Finally, we highly recommend performing a dose-response test at the beginning to decide the level in which the contamination is prevented and, importantly, the cultured cells and tissues are in their high-performance
Formulation
Please find the formulation here :Formulation
References
- Antibacterial action of penicillin, penicillin X, and streptomycin on Hemophilus influenzae. HEWITT WL, PITTMAN M. Public Health Rep (1896). 1946 May 31;61:768-78. PMID: 20983031.
- In vitro and in vivo studies of streptomycin-resistant, penicillin-susceptible streptococci from patients with infective endocarditis. Enzler MJ, Rouse MS, Henry NK, Geraci JE, Wilson WR. J Infect Dis. 1987 May;155(5):954-8. doi: 10.1093/infdis/155.5.954. PMID: 3644854.
Learn more: What Is Pen-Strep?
Additional information
| Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Frequently asked questions
What working concentration should I use for Penicillin/Streptomycin Antibiotic Mix (100X)?
Most antibiotic mixes are supplied as a 100X stock, giving a 1 in 100 dilution into complete medium. Check the Certificate of Analysis for the specific lot, since concentration varies between formulations.
Should antibiotics be used routinely in cell culture?
Most quality guidance advises against it. Continuous use masks low level contamination, allows poor aseptic technique to go unnoticed, and can select for resistant organisms. They are appropriate for high risk work such as primary tissue rather than as a standing habit.
Will this prevent yeast or fungal contamination?
Not unless it contains an antimycotic. Penicillin and streptomycin act on bacteria only. Preventing fungi or yeast requires amphotericin B or a similar antimycotic, and even then technique matters more than chemistry.
How should Penicillin/Streptomycin Antibiotic Mix (100X) be stored?
Frozen at minus 20 degrees C and aliquoted on first thaw. Activity declines with repeated freeze and thaw cycles, which shows up as contamination creeping back into cultures that were previously clean.
Is it sterility and endotoxin tested?
Yes. Every lot is filtered and tested, and a Certificate of Analysis is issued per lot and available on request.
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