Stem Cell Cryoprotective Media (SCCM-D) With DMSO

Description

Background

Certainly, there has been a development in the cryopreservation of several stem cells. Especially in  hematopoietic mesenchymal stromal and induced pluripotent stem cells.  Post-thaw cell death remains, however, a considerable problem in stem cell technology. Moreover, Stem Cell Cryoprotective Media (SCCM-D) With DMSO considerably limits several current issues. Those include the cell damage during freezing and ensure the recovery, survival, and viability as well as functionality after thawing.

Why Cryopreservation

During cryopreservation, cooling aqueous solutions shift below the equilibrium freezing point. This will subsequently result in the formation of ice crystals in the extracellular media. This  will cause a decrease in concentration of water which has two key consequences:

1. Generally, osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
2. Consequently, ice formation and abnormal concentration of solutes in the intracellular matrix, osmotic shock, and toxicity.

With decades of expertise in stem cell biology, biochemistry, and cell culture, PurMa Biologics has developed a next-generation cryoprotective media specialized for delicate stem cell culture technology. PurMa™ SCCM-D contains DMSO and gives the best preservation for stem cells, particularly proven successfully on hematopoietic, mesenchymal stromal, and induced pluripotent stem cells.

Special Features

• Ready-to-Use.
• Moreover, PurMa™ SCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.
• PurMa™ SCCM-D With DMSO provides much higher cell viability after short/long-term cryoprotection, (66% recovery rate)

• PurMa^TM SCCM-D prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and results in less exposure to toxins from the non-recovering cells.
• Preserves morphological appearance as a standard factor of cell recovery.
• We test PurMa™ SCCM-D for pH, osmolality, sterility, and endotoxin.
• Each Lot of PurMa^TM SCCM-D goes through several important tests. Those are  a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.
• PurMa^TM SCCM-D has been tested successfully for several commonly used stem cells. The most commonly used include hematopoietic, mesenchymal stromal/stem, and induced pluripotent stem cells.
• Suitable for clinical applications.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

1. Long-term preservation of permanent human hematopoietic cell lines. Mizrahi et al. J Med. 1971; 2(6) :380-6.
2. Survival of human bone marrow progenitor cells after freezing: improved detection in the colony-formation assay. Schlunk et al. 1981 Apr;18(2):111-8. doi: 10.1016/0011-2240(81)90082-1

Learn more: What Is Cryoprotective Media?