Stem Cell Cryoprotective Media (SCCM); DMSO Free

Description

Background

PurMa™ Stem Cell Cryoprotective Media (SCCM); DMSO Free lacks DMSO or other penetrating agent. PurMa™ SCCM is a powerful highly evaluated cryoprotective media. Moreover, PurMa™ SCCM has been designed to have an excellent performance in absence of any penetrating agent such as DMSO.

To emphasize, DMSO-free cryoprotective media needs to be applied only to certain cell lines which are damaged or poorly performing in the presence of DMSO or other penetrating agents.

limitations and disadvantages of DMSO

1. Basically, it has a broad toxicity.
2. Additionally, it modifies the expression of cells such an NK and T cells
3. It alters the mRNA level of the de novo DNA methyltransferase DNMT3A. This, therefore, is accompanied by hyper- or hypo-methylation of many genetic loci.
4. Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.
5. It damages the mitochondria in certain cell culture models such as astrocytes.
6. It negatively impacts cellular membrane/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.
7. Besides, it induces unwanted stem cell differentiation.
8. Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated usage of DMSO even at sub-toxic levels.
9. Afterwards, it is necessary to remove DMSO by intensive washing. That in turn results in cell sensitivity to osmotic/mechanical stresses occurring. This is a costly and time-consuming process during freezing and thawing, and moreover leads to a significant loss of cell number.

PurMa™ DMSO Free Stem Cell Cryoprotective Media provides high post-thaw cell viability and preserves original morphology, proliferation, and differentiation potentials of tested stem cells such as MSCs.

Special Features

• Ready-to-Use.
• PurMa^TM DMSO Free SCCM provides much higher cell viability after short/long-term cryoprotection, (61% recovery rate)
• PurMa^TM DMSO Free SCCM Prevents the formation of crystals in extracellular space, consequently, preventing cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
• Preserves morphological appearance as a standard factor of cell recovery.
• Tested for pH, osmolality, sterility, and endotoxin.
• Each Lot of PurMa^TM SCCM besides the pathogens and sterility evaluations goes through a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.
• PurMa^TM DMSO Free SCCM has been tested successfully for several commonly used stem cells including hematopoietic, mesenchymal stromal/stem, and induced pluripotent stem cells.
• Suitable for clinical applications.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

1. Evaluation of polyvinylpyrrolidone as a cryoprotectant for adipose tissue-derived adult stem cells. Thirumala et al. Tissue Eng Part C Methods. 2010 Aug;16(4):783-92. doi:10.1089/ten .TEC.2009.0552.
2. Long-term preservation of permanent human hematopoietic cell lines. Mizrahi et al. J Med. 1971; 2(6) :380-6.

Learn more: What Is Cryoprotective Media?