PurMa Williams' E Medium MAX
  • ✓Proudly made in the USA, manufactured in house
  • ✓Certificate of Analysis available for every lot
  • ✓Backed by decades of cell culture and protein biochemistry expertise
  • ✓Bulk pricing, purchase orders, and custom formulations welcome
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SKU: Select options below to see it Categories: Cell Culture Media, William's E Medium Tags: Cell Culture, glucose, L-Alanyl-L-Glutamine, Mammalian Cells, MAX, Phenol Red, Without Phenol Red

Williams’ E Medium – Max

(1 customer review)

Price range: $40.29 through $1025.23

Williams’ E Medium – Max contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life while significantly improving cell viability and growth. We manufacture several types of Williams’ E Medium based on your requirements.

SKU Select options below to see it

For research use only. Not for human, veterinary or diagnostic use.

Description

Background

Williams’ E Medium – Max is a commonly used media for Mammalian cell culture.

Advantages

The advantages of Williams’ E Medium – Max over Williams’ E Medium are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and is gradually cleaved by aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Williams’ E Medium – Max contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of William’s E Medium based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Williams’ E Medium – Max contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation click here: Williams’ E Medium-MAX Formulation

References

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Learn more: What Is William’s E Medium?

Additional information

Condition

William’s E -MAX Standard Formulation, William’s E -MAX w/o Phenol Red, William’s E -MAX w/o Sodium Bicarbonate, William’s E -MAX w/o Sodium Pyruvate, William’s E -MAX w/o Phenol Red, w/o Sodium Bicarbonate, William’s E -MAX Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

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East Tennessee State University Verified customer

1 review for Williams’ E Medium – Max

  1. Verified Customer, University of Oklahoma –

  2. Verified Customer, University of Oklahoma –

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Frequently asked questions

How should I store Williams’ E Medium – Max?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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