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Williams’ E Medium – Max
Williams’ E Medium – Max contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life while significantly improving cell viability and growth. We manufacture several types of Williams’ E Medium based on your requirements.
Description
Background
Williams’ E Medium – Max is a commonly used media for Mammalian cell culture.
Advantages
The advantages of Williams’ E Medium – Max over Williams’ E Medium are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and is gradually cleaved by aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
Williams’ E Medium – Max contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of William’s E Medium based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
Williams’ E Medium – Max contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
For complete formulation click here: Williams’ E Medium-MAX Formulation
References
- Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, Weisburger EK, Weisburger JH. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8. PMID:
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss TL, Sirbasku DA. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
- Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is William’s E Medium?
Additional information
| Condition | William’s E -MAX Standard Formulation, William’s E -MAX w/o Phenol Red, William’s E -MAX w/o Sodium Bicarbonate, William’s E -MAX w/o Sodium Pyruvate, William’s E -MAX w/o Phenol Red, w/o Sodium Bicarbonate, William’s E -MAX Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), William’s E -MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), William’s E -MAX 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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Frequently asked questions
How should I store Williams’ E Medium – Max?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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