MEM Alpha Modification (MEM-α) in Hanks’ Buffer
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is suitable for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, eliminating calcium in this media facilitates the growth of cells in suspension cultures.
Ingredients
MEM -α has improved regular MEM as it contains:
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
- Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
PurMa™ MEM -α media in Hank’s buffer includes HBSS
Composition of HBSS buffer contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
- Low level of glucose (1.00 g/ liter)
Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.
Application
Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent choice for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.
Formulation
to get access to detailed formulation visit ” formulation Tab” as well as by clicking here : MEM-alpha Formulation in Hank’s Buffer (HBSS)
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM-α (HBSS) Standard Formulation, MEM-α (HBSS) w/o Glutamine, MEM-α (HBSS) w/o Phenol Red, MEM-α (HBSS) w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glucose, MEM-α (HBSS) w/o Lipoic Acid, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α (HBSS) 15 mM HEPES (3.6 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), MEM-α (HBSS) w/o Sodium Pyruvate, MEM-α (HBSS) High Glucose (4.5 g/L), MEM-α (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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