- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X)
PurMa™ Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X) generally prevents contamination of the four main pathogens in cell culture. Those are bacteria, mold/ yeast, virus as well as mycoplasma. Moreover, when cells divide in media, it carries contamination along to the daughter cells. Additionally, low fungicide antibiotic mix suites cells show sensitivity to higher amount of Amphotericin B.
Description
Background
PurMaTM Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X) generally prevents contamination of the four main pathogens in cell culture. Those are bacteria, mold/ yeast, virus as well as mycoplasma. Moreover, when cells divide in media, it carries contamination along to the daughter cells. Additionally, low fungicide antibiotic mix suits cells that show sensitivity to higher amounts of Amphotericin B.
Ingredients
| Streptomycin | 10 mg/mL |
| Penicillin | 10,000 UI/mL |
| Amphotericin B | 2.5 µg/mL |
Special Features
- Cost-effective.
- Effective against both gram-positive and gram-negative bacteria.
- Contains antibiotics and antimycotics to prevent mycoplasma, fungi, and yeast.
- Additionally, it is designed to be harmless to a healthy cell line if it is used in the recommended concentration.
- More importantly, it does not cause toxicity for all the available cell lines.
- Highly recommend performing a dose-response test at the beginning to decide the level at which the contamination is prevented and at the same time cultured cells and tissues are in their high-performance
Formulation
For the formulation, Click here:Formulation
References
- Risk factors for amphotericin B-induced nephrotoxicity. Luber et al. J Antimicrob Chemother. 1999 Feb;43(2):267-71. doi: 10.1093/jac/43.2.267. PMID: 11252333.
- Screening of drugs for rapid activity against Trypanosoma cruzi trypomastigotes in vitro. Croft et al.Trop Med Parasitol. 1988 Jun;39(2):145-8.
Additional information
| Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
Frequently asked questions
What working concentration should I use for Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X)?
Most antibiotic mixes are supplied as a 100X stock, giving a 1 in 100 dilution into complete medium. Check the Certificate of Analysis for the specific lot, since concentration varies between formulations.
Should antibiotics be used routinely in cell culture?
Most quality guidance advises against it. Continuous use masks low level contamination, allows poor aseptic technique to go unnoticed, and can select for resistant organisms. They are appropriate for high risk work such as primary tissue rather than as a standing habit.
Will this prevent yeast or fungal contamination?
Not unless it contains an antimycotic. Penicillin and streptomycin act on bacteria only. Preventing fungi or yeast requires amphotericin B or a similar antimycotic, and even then technique matters more than chemistry.
How should Antibiotic Mix Solution (Low Fungicide 2.5 µg/ml) (100X) be stored?
Frozen at minus 20 degrees C and aliquoted on first thaw. Activity declines with repeated freeze and thaw cycles, which shows up as contamination creeping back into cultures that were previously clean.
Is it sterility and endotoxin tested?
Yes. Every lot is filtered and tested, and a Certificate of Analysis is issued per lot and available on request.
Related products
-
- Sale!
- Mycoplasma Detection and Elimination Reagents
Mycoplasma Growth Media
- Price range: $81.69 through $319.36
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Antibiotics
Eukaryotic Cell Culture Suited Antibiotics
- Price range: $14.30 through $101.70
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Antibiotics
Penicillin/Streptomycin Antibiotic Mix (100X)
- Price range: $29.49 through $533.89
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews