Cell Culture Blog & Laboratory Guides

Ham's F-12K (Kaighn's modification of Ham's F-12) is a nutrient-rich medium designed for demanding cell lines and low-serum culture. It builds on Richard Ham's original F-12 formulation, created for the…

McCoy's 5A is a versatile, nutrient-rich medium that supports an unusually wide range of primary cells and established cell lines. Originally developed by Thomas McCoy, its modified form (McCoy's 5A…

IMDM (Iscove's Modified Dulbecco's Medium) is a nutrient-rich basal medium built for fast-growing, high-density cultures, especially cells of the blood and immune system. It is a modification of DMEM developed…

RPMI 1640 is one of the most widely used cell culture media, especially for cells grown in suspension. Developed at Roswell Park Memorial Institute, it was designed originally for human…

DMEM (Dulbecco's Modified Eagle's Medium) is the most widely used basal medium in cell culture. It is a richer version of Eagle's Minimal Essential Medium, developed by Renato Dulbecco, and…

Bovine calf serum (BCS), sometimes just called calf serum, is a widely used and cost-effective supplement for cell culture media. It is collected from the blood of young calves and…

Trypsin is one of the most-used reagents in any cell culture lab. It is the enzyme researchers reach for to detach adherent cells from their culture vessel so they can…

EMEM (Eagle's Minimal Essential Medium) is one of the most widely used basal media in cell culture. Developed by Harry Eagle as a refinement of his earlier Basal Medium Eagle,…

Horse serum (HS) is the liquid fraction of horse blood that remains after clotting, once the cells and clotting factors are removed. In the lab it is used as a…

DMEM vs DMEM F12 is a common fork in the road once a cell line needs more than a basic Eagle-style medium can offer. Both share the same DMEM backbone,…