A Practical Guide to the Liquid Nitrogen Dewar
A liquid nitrogen dewar is a specialized, vacuum-insulated container designed to hold and transport liquid nitrogen (LN2) at its incredibly low temperature of -196°C (-320°F). You can think of it…
Cell Culture Blog & Laboratory Guides
Practical protocols, troubleshooting guides, media comparisons, and laboratory resources from PurMa Biologics.
0.22 Micron Filter: The Complete Guide to Sterile Filtration
The 0.22 micron (0.22 µm) filter is an essential tool for sterile filtration. It’s what stands between your meticulously prepared cell culture media and the microbial chaos of the outside…
Your Definitive 10x PBS Recipe for Flawless Cell Culture
A perfected 10x PBS recipe is the bedrock of reproducible science. It's not just about convenience; a reliable stock solution is your first line of defense for consistency across every…
ACK Lysing Buffer: RBC Removal Protocol and Troubleshooting
ACK (Ammonium-Chloride-Potassium) lysing buffer is built for a single, non-negotiable task in the lab: getting rid of red blood cells (RBCs). When you need a clean population of nucleated cells—like…
Unlocking the ecor1 restriction site: A Clear Guide to EcoRI’s Power
In the world of molecular biology, some tools are so fundamental they're like the alphabet. The EcoRI restriction site is one of them. It’s a specific DNA sequence—5'-GAATTC-3'—that acts as…
Mastering the ecor1 restriction enzyme: A Scientist’s Guide
The EcoRI restriction enzyme is one of those tools you can’t imagine modern molecular biology without. Think of it as a pair of molecular scissors, but with a critical difference:…
Decoding Your Gene Expression Profile A Practical Guide
If you think of your DNA as a massive library holding thousands of recipe books (your genes), then a gene expression profile is a list of which books are currently…
His Tag Protein Purification: Protocol, Buffers and Troubleshooting
His tag protein purification pulls one recombinant protein out of a crude cell lysate in a single step. You add a short string of histidines to the protein, let it…
A Practical Guide to In Vitro Transcription
In vitro transcription (IVT) is a cell-free method used to synthesize large quantities of specific RNA molecules from a DNA template in a test tube. Think of it as a…
Transwell Migration Assay: A Researcher’s Protocol Guide
A transwell migration assay is a lab workhorse for a reason. It's an elegant, straightforward method for watching and measuring how cells move, using a simple insert with a porous…
