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MEM α-Modification with Nucleosides in Hanks’ Buffer

Background

MEM α-Modification with Nucleosides  in Hanks’ buffer provides a great nutritional culture environment during mammalian cells.  This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.

Ingredients

MEM-α with nucleosides is basically the modified version of MEM-α and contains:

• Ribonucleosides
• Deoxyribonucleosides
• Non-essential amino acids.
• Sodium pyruvate.
• Lipoic acid.
• Vitamin B12.
• Biotin and,
• Ascorbic acid.

PurMa™ MEM-α-N-HBSS contains HBSS buffer

The composition of HBSS buffer:

1. Potassium Chloride
2. Potassium Phosphate, monobasic
3. Sodium Bicarbonate
4. Sodium Chloride
5. Sodium Phosphate, dibasic

Additionally, MEM-α-N-HBSS also Contains:

• Low level of glucose (1.00 g/ liter)
• Sodium bicarbonate (0.35 g/L)
• 2 mM L-glutamine (292 mg/L)

PurMa Biologics manufactures 119 types of MEM-α-N-HBSS

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Application

Furthermore,  MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.

Formulation

For complete formulation  click here as well as " formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation

References

1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.  Keay L, Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""] [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more