Eagle’s Minimum Essential Medium (EMEM)

Background

Eagle’s Minimal Essential Medium (EMEM) was developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Several versions of EMEM have been developed by modification of its composition. This media is suitable for a wide range of mammalian cells – adhesive as well as suspension cells.

Ingredients

Moreover, Eagle’s Minimal Essential Medium (EMEM) Medium contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (1.5 g/L)
  • Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
  • 2 mM L-glutamine (292 mg/L)
  • 1mM sodium pyruvate (0.11g/L)
  • Additionally, a higher concentration of amino acids, vitamins, inositol, and choline are present in this medium.

PurMa Biologics manufactures 106 types of EMEM which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for EMEM media.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting-edge cell and tissue culture science.

We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.

We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.

Considering the highest quality, our prices are extremely reasonable.

Application of EMEM

Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cell lines. The dedicated scientists at PurMa Biologics have decades of experience with this media for culturing primary in addition to commercial cell lines, including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.

Formulation

Click here for the complete formulation:  EMEM Formulation