Chemically Defined DMEM

Chemically Defined (CD) DMEM

(36 customer reviews)

PurMa™ Chemically Defined DMEM is a proprietary formulated Medium which does NOT need to be supplemented with Serum  and the growth of cells is equivalent of media + 10 % FBS

SKU: P3A210105 Categories: Cell Culture Media, Dulbecco’s Modified Eagle’s Medium (DMEM) Tags: Advanced, Cell Culture, DMEM, Dulbecco's, Human Transferrin, L-Alanyl-L-Glutamine, Recombinant Insulin, Serum Reduction

Description

Background:

Over the past three decades, PurMa Biologics has tenaciously worked to develop fully synthetic cell culture media by eliminating any animal products. We are proud to have developed a chemically defined forms of all the conventional media such as DMEM, DMEM/F12, IMDM, RPMI-1640. The PurMaTM CD supplement cocktail containing specific growth factors, hormones, or nutrients varies based on cell type and other factors such as other ingredients in the medium.

CD-DMEM Contains:

PurMaTM Chemically Defined DMEM is a proprietary formulated used to grow cells in vitro without any undefined or animal-derived components.Moreover,  CD-DMEM contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.

This provides a consistent and controlled environment for cell growth and research. Noticeably, CD-DMEM is essential for applications requires reproducibility and regulatory compliance as well as for studying specific cellular processes. without extraneous variables. Furthermore, CD-DMEM is designed for custom formulations tailored for various cell types

Inportant Note:

It is crucially vital for a graduate transition from media containing FBS to chemically defined cell culture media, follow these steps:

  • Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMaTM Chemically Defined Medium. The second one chemically defined medium + 10% FBS which is going through the reduction of FBS process
  • 48 hours later: 8% FBS in + PurMaTM Chemically Defined Medium
  • 48 hours later: 6% FBS in + PurMaTM Chemically Defined Medium
  • 48 hours later: 4% FBS+ PurMaTM Chemically Defined Medium
  • 48 hours later: 2% FBS+ PurMaTM Chemically Defined Medium
  • 48 hours later: 1 FBS and + PurMaTM Chemically Defined Medium
  • 24 hours later: 0$+% FBS and only PurMaTM Chemically Defined Medium

Importantly, for each change, spin down and fully discard  the media and replace it with the less percentages of FBS.

Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated

So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).

Usually, after you get your cells adopted to PurMaTM Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adopted to chemically defined medium and when you thaw them in the first week, in addition to PurMaTM Chemically Defined Medium, you need to add 10% PurMaTM CD Booster (Cat# P3C10144).

CD-DMEM Formulation:

The CD DMEM includes the the max formulation of DMEM including L-Alanine -L glutamine :  DMEM MAX Formulation

Additionally , CD-DMEM contains a proprietary synthetic cocktail of specific growth factors, hormones, and other  nutrients which makes it independent of any serum

References:

  1. Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells. Kolkmann AM. et al, 2022, 4;10:895289
  2. CNTF+BDNF treatment and neuroprotective pathways in the rd1 mouse retina. Azadi, S. et al, 2007, 19;1129(1):116-29

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 292-335 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.1 µm,  filter 3times
Form Liquid
Shipping Condition  Room temperature, Overnight

 

Additional information

Condition

CD-DMEM – Standard Formulation, CD-DMEM – Without L-Alanyl-L-Glutamine, CD-DMEM – Without Phenol Red, CD-DMEM – Without Sodium Bicarbonate, CD-DMEM – Without Glucose, CD-DMEM – Low Glucose (1.5 g/L), CD-DMEM – Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – 15 mM HEPES (3.6 g/L), CD-DMEM – 25 mM HEPES (5.9 g/L), CD-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, CD-DMEM – Without L-Alanyl-L-Glutamine, Without Sodium Bicarbonate, CD-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Bicarbonate, CD-DMEM – 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), CD-DMEM – Without Phenol Red, Without Sodium Bicarbonate, CD-DMEM – Without phenol Red, 15 mM HEPES (3.6 g/L), CD-DMEM – Without phenol Red, 25 mM HEPES (5.9 g/L), CD-DMEM – Without phenol Red, Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid
Size

1X 100 ml, 1X 1000 ml, 1X 500 ml

36 reviews for Chemically Defined (CD) DMEM

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

  19. Surendra-Shukla@ouhsc.edu –

    Review: Very fast, efficient, and economic service. Great for new PIs like me.
    Have you purchased: Yes
    Time of submission: 25-Apr-2025 14:00:04
    Ease of placing the order: 5/5

  20. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  21. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  22. anil-singh@ouhsc.edu –

    Review: NA
    Have you purchased: Yes
    Time of submission: 25-Mar-2025 10:11:14
    Ease of placing the order: 5/5

  23. Surendra-Shukla@ouhsc.edu –

    Review: Best quality products, best price.
    Have you purchased: Yes
    Time of submission: 18-Apr-2025 09:51:03
    Ease of placing the order: 5/5

  24. madhusmita-rout@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 21-Apr-2025 11:15:46
    Ease of placing the order: 5/5

  25. alexholloway@mesogen.com –

    Review: This serum appears to be a high quality product and worked well for our needs. Purma has an extensive catalog for cell culturing supplies which we are glad we came across.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Apr-2025 16:54:29
    Ease of placing the order: 5/5

  26. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  27. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  28. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  29. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  30. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  31. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  32. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  33. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  34. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  35. Ravi-Gor@ouhsc.edu –

    Review: I recently had the pleasure of interacting with Dr. Seif Azadi, and I want to express my gratitude for his expertise and timely assistance. His knowledge and dedication were instrumental in addressing our lab’s needs efficiently.
    Have you purchased: It is my first Purchase
    Time of submission: 25-Jun-2025 12:00:09
    Ease of placing the order: 5/5

  36. xiangpeng-li@ou.edu –

    Review: PurMa helped us with the customized medium. They are professional and friendly. I appreciate their great work.
    Have you purchased: Yes
    Time of submission: 14-Aug-2025 12:59:31
    Ease of placing the order: 5/5

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