Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM)

Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) needs 2-5% FBS and provides more consistency.

Description

Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM)

Background:

PurMaTM Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes.  In Advanced DMEM, L-Alanyl-L-Glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

A-DMEM Contains:

  • Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) contains all the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Additionally, A-DMEM is a significant step toward a chemically defined form of this media. Undoubtedly, Chemically Defined DMEM (CD-DMEM) is the outcome of decades of cutting-edge research. CD-DMEM will soon be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is available here: Advanced DMEM Formulation

References:

  1. A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
  2. Protective layer formation on magnesium in cell culture medium. Wagener V, Virtanen S.Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
  3. The viability change of pigskin in vitro. Ge L, Sun L, Chen J, Mao X, Kong Y, Xiong F, Wu J, Wei H. 2010 Jun;36(4):533-8. doi: 10.1016/j.burns.2009.08.001.PMID: 19836142.Additionally

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-DMEM- Standard Formulation, A-DMEM- Without L-Alanyl-L-Glutamine, A-DMEM- Without Phenol Red, A-DMEM- Without Sodium Bicarbonate, A-DMEM- Without Sodium Pyruvate, A-DMEM- Without Glucose, A-DMEM- With Lipoic Acid, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, A-DMEM- Without L-Alanyl-L-Glutamine, Without Sodium Bicarbonate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Sodium Pyruvate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Bicarbonate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Pyruvate, A-DMEM- Without Sodium Bicarbonate, Without Sodium Pyruvate, A-DMEM- Low Glucose (1.5 g/L), A-DMEM- Low Sodium Bicarbonate (1.5 g/L), A-DMEM- 25 mM HEPES (5.9 g/L), A-DMEM- 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, A-DMEM- Without Phenol Red, Without Sodium Bicarbonate, A-DMEM- Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), A-DMEM- Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), A-DMEM- Without phenol Red, 15 mM HEPES (3.6 g/L), A-DMEM- Without phenol Red, 25 mM HEPES (5.9 g/L), A-DMEM- Without phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced DMEM Formulation 

 

SDS

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