Description

Charcoal-Stripped Fetal Bovine Serum (CS-FBS)

Background

Charcoal-Stripped Fetal Bovine Serum (CS-FBS) is the outcome of treating the Fetal Bovine Serum or Calf serum with activated carbon removes hormones and steroids from FBS.

• This process removes all the non-polar material such as lipophilic (lipid-related) materials (viruses, certain growth factors, hormones, and cytokines).
• Acts as an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads in order to prevent nonspecific binding).
• Moreover, it performs the function regardless of the molecular weight of the target.

PurMa Biologics applying Norit A charcoal coated with Dextran T-70 which manufactures a high-quality Charcoal-Stripped Fetal Bovine Serum.

PurMa™ Charcoal-Stripped FBS (DFBS)

• PurMa^TM Premium FBS is USA origin.
• Goes through Heat in activation and  gamma irradiation.
• Following the stripping, the FBS goes through heat inactivation process.
• PurMa Biologics perform vigorous analysis following the stripping and will provide a certificate of analysis.
• The procedure depletes any residue of  mycoplasma, steroids and hormones, and other pathogens.
• Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
• Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to make sure cultured cells are well beings.

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

Further Instructions

On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.

References

1. The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. Lippman et al. Cancer Res. 1976 Dec;36(12):4595-601. PMID:
2. Leake et al. Steroid Hormones: A Practical Approach. Oxford, UK: IRL Press; 1987.
3. Effects of estrogens and antiestrogens on estrogen receptor dynamics and the induction of progesterone receptor in MCF-7 human breast cancer cells. Eckert et al. Cancer Res. 1982 Jan;42(1):139-44.