- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Dialyzed Fetal Bovine Serum (D-FBS)
Fetal bovine serum dialyzed to remove small molecules, for experiments that need a more defined culture environment.
- Reduces low-molecular-weight components: antibiotics, nucleotides, amino acids, and hormones
- Improves consistency for defined-media and metabolic studies
Description
Background
Dialyzed Fetal Bovine Serum (D-FBS) provides more consistency among the cell culture experiments. That especially stands out in studies requiring a more defined environment of small molecules. Moreover, the dialysis process eliminates/reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, as well as various small proteins in the serum.
PurMaTM Dialyzed FBS is Free of Tetracycline and other Antibiotics.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMaTM Dialyzed FBS (DFBS)
- PurMaTM Premium FBS is of USA origin.
- We perform the dialysis using the 3500 Dalton (3.5 kDa) dialysis tube. The procedure carries out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FBS is sterilized by 0.1 µM filters three times.
- The parent FBS has gone through gamma irradiation as well as heat inactivation process.
- PurMa Biologics performs vigorous analysis following the dialysis, which results in a certificate of analysis.
- Free of Mycoplasma, steroids and hormones, and other pathogens.
- Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
- Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFBS in your media to ensure cultured cell wellbeing.
Analysis
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
Further Instructions
On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.
References
- Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. van der Valk et al. Toxicol In Vitro. 2010 Jun;24(4):1053-63. doi: 10.1016/j.tiv.2010.03.016. Epub 2010 Mar 31. PMID: 2036204.
- Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology.Brovkovych et al. Proteomics; available in PMC 2019 Sep 12.Published in final edited formas: Proteomics. 2019 Sep; 19(18): e1800370. Published online 2019 May 7. doi: 10.1002/pmic.201800370. PMCID: PMC6742575.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing (9 CFR 113.53c) | |||
| Bovine Adenovirus | NA | Not Detected | Not Detected |
| Bovine Parvovirus | NA | Not Detected | Not Detected |
| Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
| Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Fetal Bovine Serum Used For?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
Frequently asked questions
What sizes does D-FBS come in?
Dialyzed Fetal Bovine Serum (D-FBS) is supplied in 50 mL and 500 mL bottles.
What does dialysis remove from the serum?
Dialysis against a 3,500 Dalton membrane removes low molecular weight components, which includes antibiotics, nucleotides, amino acids, hormones, salts and small proteins. The larger growth factors and proteins remain, so the serum still supports growth but in a much more defined background.
When should I use dialyzed FBS?
Use it when small molecules in standard serum would confound the experiment: metabolic labelling, nutrient limitation and selection studies, defined media work, and assays sensitive to serum nucleotides or amino acids. Because dialysis removes some small growth factors, 15 to 20% D-FBS is often recommended in place of the usual 10%.
Is D-FBS tested, and is a Certificate of Analysis available?
Yes. It is manufactured in house in the USA and each lot ships with a Certificate of Analysis available on request. Lot specific storage and expiry are printed on the bottle and listed on the COA.
Do you offer bulk pricing and purchase orders for D-FBS?
Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.
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