Description

Dialyzed Fetal Bovine Serum (D-FBS)

Background

Dialyzed Fetal Bovine Serum (D-FBS) provides more consistency among the cell culture experiments. That especially stands out in studies requiring a more defined environment of small molecules. moreover, The dialysis process eliminates/ reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, as well as various small proteins in the serum.

PurMa^TM Dialyzed FBS is Free of Tetracycline and other Antibiotics.

PurMa^TM Dialyzed FBS (DFBS)

• PurMa^TM Premium FBS is USA origin.
• We perform the dialysis using the 3500 Dalton (3.5 kDa) dialysis tube. The procedure carries out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FBS sterilized  by 0.1 µM filters for three times.
• The parent FBS Has gone through gamma irradiation as well as heat inactivation process.
• PurMa Biologics performs vigorous analysis following the dialysis which results in  and certificate of analysis.
• Free of Mycoplasma, steroids and hormones, and other pathogens.
• Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
• Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFBS in your media to make sure of cultured cell well beings.

Analysis

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

Further Instructions

On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.

References

1. Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. van der Valk et al. Toxicol In Vitro. 2010 Jun;24(4):1053-63. doi: 10.1016/j.tiv.2010.03.016. Epub 2010 Mar 31. PMID: 2036204.
2. Removal of serum lipids and lipid-derived metabolites to investigate breast cancer cell biology.Brovkovych et al. Proteomics; available in PMC 2019 Sep 12.Published in final edited formas: Proteomics. 2019 Sep; 19(18): e1800370. Published online 2019 May 7. doi: 10.1002/pmic.201800370. PMCID: PMC6742575.