- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Lipid-Depleted Fetal Bovine Serum (LD-FBS)
Fetal bovine serum with lipids removed, for studies where serum lipids would confound results.
- Best for lipid-metabolism research and lipid-sensitive assays
- Removes lipids that can affect key cellular checkpoints
- Enables preparation of media with defined lipid species
Description
Background
The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture is carried out in the presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best uses for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications are:
- When the normal concentration of lipids may interfere with the results.
- For studies that directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.
- It is used for the generation of media with defined lipid species.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
Special Features
- PurMaTM Premium LD-FBS is of USA origin
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- PurMa Biologics performs the most sophisticated lipid depletion.
- The process will continue with dialysis using the 3500 Dalton dialysis tube.
- The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.
- The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.
- Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.
- The process eliminates mycoplasma, IgG, steroids and hormones, and other pathogens.
- Acceptable level of hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).
Analysis
An example of results following PurMa™ FBS Lipid depletion procedure : The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion
For non-proprietary information in PurMa biologics’ general procedure for FBS Lipid depletion, click here: PurMa™ FBS Lipid depletion Procedure
Routine pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Effect of the removal of lipids on specific precipitation. Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383–391. doi: 10.1084/jem.92.5.383. PMCID: PMC2135985.
- Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture. Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769/Bio Pro toc.2876, PMCID: PMC8275250.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing (9 CFR 113.53c) | |||
| Bovine Adenovirus | NA | Not Detected | Not Detected |
| Bovine Parvovirus | NA | Not Detected | Not Detected |
| Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
| Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Fetal Bovine Serum Used For?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
3 reviews for Lipid-Depleted Fetal Bovine Serum (LD-FBS)
Frequently asked questions
How should I store and thaw Lipid-Depleted Fetal Bovine Serum (LD-FBS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
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Verified Customer –
we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Michael Rudolph, OU Health Sciences Center –
I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Rohan Varshney, OU Health Sciences Center –
Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.