- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
Fetal bovine serum with endotoxin reduced to very low levels for endotoxin-sensitive work.
- Triple filtered at 0.1 µm, then processed through PurMa’s Endotoxin Elimination Column
- Each lot evaluated for endotoxin
- Ideal where residual endotoxin can interfere with results
Description
Background
PurMa Biologics manufactures seven types of bovine serum, three of which, however, are considered premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover, We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm, the residual endotoxin was depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMaTM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
- PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- Lastly, we vigorously analyze various aspects and each LOT possesses a certificate of analysis.
Endotoxin Removal Procedure
- PurMa Biologics processes PurMaTM ED-FBS from PurMaTM Premium Fetal Bovine Serum (P-FBS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
- The process of removing endotoxin from FBS routinely starts with passing the premium FBS through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) at the rate of 0.1 ml/minute.
- The unbound fractions pass through 0.1µm filters three times. The mentioned procedure eliminates the residual of endotoxin as well.
- We conjugate Sepharose 6B in our proprietary column covalently to a powerful endotoxin-specific absorbent.
Analysis
PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Biochemical and morphological studies of steady state and lipopolysaccharide treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
- The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | Not Detected | Not Detected |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing (9 CFR 113.53c) | |||
| Bovine Adenovirus | NA | Not Detected | Not Detected |
| Bovine Parvovirus | NA | Not Detected | Not Detected |
| Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
| Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Fetal Bovine Serum Used For?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Frequently asked questions
How should I store and thaw Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
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