Dialyzed Fetal Horse Serum (DFHS)
Fetal horse serum dialyzed to remove small molecules, for experiments that need a more defined environment.
- Reduces low-molecular-weight components for defined-media studies
- Improves consistency across cell culture experiments
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
Animal serum contains many undefined components. PurMa™ Dialyzed Fetal Horse Serum (DFHS) provides more consistency among the cell culture experiments. This is especially true in studies requiring a more defined environment of small molecules. The dialysis process eliminates/ reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, and various small proteins in serum.
PurMaTM Dialyzed FHS is Free of Tetracycline and other Antibiotics.
PurMa™ Dialyzed FHS (DFHS)
- DFHS has been dialyzed using the 3500 Dalton (3.5 kDa) dialysis tube. The dialysis is carried out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FHS is filtered three times (0.1 µM filters).
- PurMa™ Dialyzed Fetal Horse Serum (DFHS) is of USA origin.
- The parent DFHS has been gamma irradiated.
- Following the dialysis, the FHS goes through heat inactivation process.
- Following the dialysis, the FHS goes through vigorous analysis and will get a certificate of analysis.
- Moreover, PurMa™ Dialyzed Fetal Horse Serum (DFHS) is free of Mycoplasma, steroids and hormones and other pathogens.
- PurMa™ DFHS is free of endotoxin and also contains an acceptable level of hemoglobin (20 mg/Dl).
- Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFHS in your media to ensure cultured cell wellbeing.
Further Instructions
On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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