- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Charcoal-Stripped Fetal Horse Serum (CS-FHS)
Fetal horse serum treated with activated charcoal to remove hormones and steroids.
- Activated-carbon stripping removes steroids and hormones
- For hormone- and steroid-sensitive horse-serum applications
Description
Background
PurMa Biologics manufactures PurMa™ Charcoal-Stripped Fetal Horse Serum (CS-FHS) by treating the Fetal Horse Serum with activated carbon, an effective process for removing hormones and steroids.
- This process removes all the non-polar material such as lipophilic (lipid-related) materials (virus, certain growth factors, hormones, and cytokines).
- It is an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads to prevent nonspecific binding).
- Regardless of the molecular weight of the target.
PurMa Biologics applies Norit A charcoal coated with Dextran T-70 to manufacture a high-quality Charcoal-Stripped Fetal Horse Serum.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMa™ Charcoal-Stripped FHS (DFHS)
- PurMaTM Premium FHS is of USA origin.
- The parent FHS has been gamma irradiated.
- Following the stripping, the FHS goes through heat inactivation process.
- Following the stripping, the FHS goes through vigorous analysis and will get a certificate of analysis.
- It is free of mycoplasma, steroids and hormones, and other pathogens.
- Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
- Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to ensure cultured cells’ wellbeing.
Further Instructions
On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Estrogen mitogenic action. I. Demonstration of estrogen-dependent MTW9/PL2 carcinogen-induced rat mammary tumor cell growth in serum-supplemented culture and technical implications. Moreno-Cuevas et al. In Vitro Cell Dev Biol Anim. 2000 Jul-Aug;36(7):410-27.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
Frequently asked questions
What sizes does CS-FHS come in?
Charcoal-Stripped Fetal Horse Serum (CS-FHS) is supplied in 50 mL and 500 mL bottles.
What does charcoal stripping remove?
Treatment with dextran coated charcoal removes small lipophilic molecules, most importantly steroid hormones, along with some growth factors and cytokines. The result is a serum with a very low hormone background while still supporting culture.
When do I need charcoal stripped serum?
Whenever background steroid or hormone activity would interfere with the readout. The standard cases are oestrogen and androgen receptor studies, nuclear receptor assays, and any experiment where you are dosing a hormone and need a clean baseline to measure against.
Is CS-FHS tested, and is a Certificate of Analysis available?
Yes. It is manufactured in house in the USA and each lot ships with a Certificate of Analysis available on request. Lot specific storage and expiry are printed on the bottle and listed on the COA.
Do you offer bulk pricing and purchase orders for CS-FHS?
Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.
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