Charcoal-Stripped Fetal Horse Serum (CS-FHS)
Fetal horse serum treated with activated charcoal to remove hormones and steroids.
- Activated-carbon stripping removes steroids and hormones
- For hormone- and steroid-sensitive horse-serum applications
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
PurMa Biologics manufactures PurMa™ Charcoal-Stripped Fetal Horse Serum (CS-FHS) by treating the Fetal Horse Serum with activated carbon, an effective process for removing hormones and steroids.
- This process removes all the non-polar material such as lipophilic (lipid-related) materials (virus, certain growth factors, hormones, and cytokines).
- It is an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads to prevent nonspecific binding).
- Regardless of the molecular weight of the target.
PurMa Biologics applies Norit A charcoal coated with Dextran T-70 to manufacture a high-quality Charcoal-Stripped Fetal Horse Serum.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMa™ Charcoal-Stripped FHS (DFHS)
- PurMaTM Premium FHS is of USA origin.
- The parent FHS has been gamma irradiated.
- Following the stripping, the FHS goes through heat inactivation process.
- Following the stripping, the FHS goes through vigorous analysis and will get a certificate of analysis.
- It is free of mycoplasma, steroids and hormones, and other pathogens.
- Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
- Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to ensure cultured cells’ wellbeing.
Further Instructions
On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Estrogen mitogenic action. I. Demonstration of estrogen-dependent MTW9/PL2 carcinogen-induced rat mammary tumor cell growth in serum-supplemented culture and technical implications. Moreno-Cuevas et al. In Vitro Cell Dev Biol Anim. 2000 Jul-Aug;36(7):410-27.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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