- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS)
PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is specifically designed to support robust and reliable cell growth by effectively removing endotoxins that can compromise cellular function. Endotoxin depletion creates a more stable and physiologically supportive environment for cell proliferation, leading to improved experimental consistency and performance.By using ED-FHS, researchers can:
- Reduce variability in cell culture conditions
- Enhance cell viability, growth, and productivity
- Improve the reliability and reproducibility of results, especially with endotoxin-sensitive cell lines and assays
PurMa™ ED-FHS represents a significant advancement for the culture of sensitive cell types, making it ideal for high-precision and endotoxin-sensitive applications.
Description
Background
PurMa Biologics manufactures seven types of horse serum, including PurMa™ Endotoxin Depleted Fetal Horse Serum (ED-FHS) each specialized for certain requirements in tissue culture:
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS).
Additionally, PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is processed from PurMa™ Premium Fetal Horse Serum (P-FHS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
Endotoxin Removal
PurMa Biologics removes the endotoxin from FHS by passing the premium FHS through the proprietary PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). The Sepharose 6B in our column is covalently conjugated to an endotoxin-specific absorbent. The mentioned procedure follows by eliminating the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
Product Features
- Firstly, PurMa™ Endotoxin Depleted Fetal Horse Serum (ED-FHS) is of USA origin.
- Also, it is free of endotoxin.
- Moreover, PurMa™ Endotoxin Depleted Fetal Horse Serum (ED-FHS) has been gamma irradiated.
- Has been heat inactivated.
- And lastly, has been vigorously analyzed with the certificate of analysis.
Routine pathological analysis on PurMa bovine and horse serums:
Reference
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
Methodology |
Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Frequently asked questions
How should I store and thaw PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
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