- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Ultra-Premium Fetal Horse Serum (UP-FHS)
Our highest-consistency fetal horse serum, processed to minimize batch-to-batch variation.
- Selected for reproducibility and lot-to-lot consistency
- Reduces variability in sensitive in vitro cell culture studies
Description
Background
Reproducibility and consistency regarding In vitro cell culture experiments is a matter of concern. The inconsistency in In vitro cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) has far less fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus. Moreover, PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) is obtained from very young donor equine serum of US origin, and with a proprietary method, the serum is isolated and purified without compromising the crucial elements for growth.
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMa Biologics has three main premium fetal horse serums
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin Depleted Fetal Horse Serum (EDP-FHS).
The Major Preference of UP-FHS Over P-FHS
- The source of the Blood and therefore
- Innovative grade US origin horse serum which are consistent among the Lots.
- Moreover, the significantly higher growth by UP-FHS compared to P-FHS
- Also, the depth of the analysis
- Besides, the parameters are evaluated. This helps with the consistency of analysis using various Lots.
PurMa Biologics routinely tests PurMa™Ultra-Premium Fetal Horse Serum (UP-FHS) for the following elements. That is, of course, besides the routine tests which are provided in the “Serum Analysis Tab.”
Routine Quality Analysis
With relative order of importance: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, Lactate Dehydrogenase, TIBC, SGOT, SGPT.
PurMa™ Ultra-Premium FHS
- All three PurMa™ Ultra-Premium FHS are, indeed, of USA origin.
- We additionally sterilize FHS by gamma irradiation.
- Have been heat inactivated.
- PurMa Biologics vigorously analyzes all bovine and horse serums and, that results in a certificate of analysis for each Lot.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
Frequently asked questions
How should I store and thaw Ultra-Premium Fetal Horse Serum (UP-FHS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
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