Ultra-Premium Fetal Horse Serum (UP-FHS)
Reproducibility and consistency regarding In vitro cell culture experiments is a matter of concern. The inconsistency in In vitro cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) has far fewer fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus.
Description
Ultra-Premium Fetal Horse Serum (UP-FHS)
Background
Reproducibility and consistency regarding In vitro cell culture experiments is a matter of concern. The inconsistency in In vitro cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) has far less fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus.
PurMa Biologics has three main premium fetal horse serums
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin Depleted Fetal Horse Serum (EDP-FHS).
The Major Preference of UP-FHS Over P-FHS
- The source of the Blood and therefore:
- Moreover, the significant higher growth by UP-FBS compared to P-FHS
- Also, the depth of the analysis
- Besides, the parameters are evaluated. This helps with the consistency of analysis using various Lots.
PurMa Biologics routinely tests PurMa™Ultra-Premium Fetal Horse Serum (UP-FHS) for the following elements. That is of course, besides the routine tests which is provided in the “Serum Analysis Tab “.
Routine Quality Analysis
With relative order of importance: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, Lactate Dehydrogenase, TIBC, SGOT, SGPT.
PurMa™ Ultra-Premium FHS
- All three PurMa™ Ultra-Premium FHS are, indeed, of USA origin.
- We additionally sterilize FHS by gamma irradiation.
- Have been heat inactivated.
- PurMa Biologics vigorously analyzes all bovine and horse serums and, that results in a certificate of analysis for each Lot.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
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