- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Horse Serum (HS)
A versatile growth supplement used in labs worldwide for routine in vitro culture of many cell types.
- Supports growth of human embryo fibroblasts and hepatitis models
- US-origin horse serum for general cell and tissue culture
Description
Background
Laboratories around the globe use Horse Serum (HS) to support the growth of many cell types In vitro. HS provides proper growth for human Embryo Fibroblast as well as models of hepatitis. Moreover, it performs well with U937 macrophage, human lymphocytes as well as blood cells including cord blood cells, T and B lymphocytes. We collect Horse Serum (HS) from foals less than 12 months old.
Horse Serum (HS) Vs. Fetal Horse Serum (FHS)
- HS contains approximately twice the protein compared to fetal horse serum (FHS), among them IgGs and other antibodies.
- Contains fewer growth factors than fetal horse serum, however, is proven to be adequate for the above-mentioned cell lines.
- Importantly, it is much more cost-effective than Fetal Horse Serum (FHS).
Special Features
- PurMa™ Horse Serum (HS) has US origin.
- PurMa™ Horse Serum (HS) has been triple-filtered through 0.1 µM filters.
- PurMa Biologics sterilizes HS with gamma irradiation methodology.
- PurMa Biologics performs heat-inactivation on our HS.
- Has been tested for mycoplasma as well as endotoxin (see below), see the certificate of analysis.
- Has been tested on multiple cell lines, such as Hek293, COS1, and NS1 cells.
Analysis
The Analysis performed on all PurMa™ Bovine and Horse Serums: Routine Pathological Analysis on PurMa™ Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8. doi: 10.1007/BF02623400. PMID:
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8. doi: 10.1007/BF02618879. PMID:
- Serum and growth factor requirements for proliferation of human adrenocortical cells in culture: comparison with bovine adrenocortical cells. Hornsby et al. In Vitro. 1983 Nov;19(11):863-9. doi: 10.1007/BF02618166. PMID:
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
Frequently asked questions
What sizes does horse serum come in?
Horse Serum (HS) is supplied in 50 mL and 500 mL bottles.
When is horse serum used instead of FBS?
Horse serum is the standard choice in several settings where bovine serum is unsuitable, including myoblast differentiation, neuronal culture and hybridoma work. It tends to be gentler and less mitogenic than FBS, which is exactly what is wanted when you are trying to push cells to differentiate rather than proliferate.
Do you also supply specialty horse serum grades?
Yes. Alongside standard horse serum, PurMa manufactures fetal horse serum in premium, ultra premium, dialyzed, lipid depleted, endotoxin depleted and charcoal stripped grades.
Is horse serum tested, and is a Certificate of Analysis available?
Yes. It is manufactured in house in the USA and each lot ships with a Certificate of Analysis available on request. Lot specific storage and expiry are printed on the bottle and listed on the COA.
Do you offer bulk pricing and purchase orders for horse serum?
Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.
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