- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Premium Fetal Horse Serum (P-FHS)
Research-grade Fetal Horse Serum (P-FHS) supplying growth factors and nutrients for mammalian cell culture.
- Derived from horse fetal blood
- Provides undefined growth factors and nutrients for eukaryotic cells
- A widely used supplement for cell culture
Description
Background
Premium Fetal Horse Serum (P-FHS) is derived from the blood of the horse fetus. Cell culture laboratories use FHS for various purposes. P-FHS contains undefined nutrition, growth factors as well. Therefore, it remains a widely used supplement for cell culture. PurMa Biologics has developed and processes three main premium fetal horse serums, each specialized for certain requirements in tissue culture.
Our Serum Products
PurMa Biologics produces seven types of horse serums. The three following are considered premium:
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin Depleted Fetal Horse Serum (EDP-FHS).
PurMa™ Premium FHS
- All three PurMaTM Premium FHS are, indeed, of USA origin.
- We additionally sterilize FHS by gamma irradiation.
- Have been heat inactivated.
- PurMa Biologics vigorously analyzes all bovine and horse serums and, that results in a certificate of analysis for each Lot.
The major differences between P-FHS and UP-FHS
- The source of the Blood and therefore:
- Significantly higher growth by Ultra-Premium Fetal Horse Serum (UP-FHS) compared to P-FHS, which determines the potency of the serum.
- The depth of the analysis:
- Quality of PurMa™ Premium Fetal Horse Serum (P-FHS)
Over the years, we have tested and analyzed PurMa™ Premium Fetal Horse Serum (P-FHS). Please see the below table.
We routinely test every Lot and evaluate the following criteria: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, , Lactate Dehydrogenase, TIBC, SGOT, SGPT.
Analysis
Click here for the detailed analysis:Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Bacteriostatic effects of horse sera and serum fractions on Clostridium welchii Type A, and the abolition of bacteriostasis by iron salts. Rogers HJ. 1967 Mar;12(3):285-301.
- Comparison of the structure of the immunoglobulins from horse serum. Weir et al. Biochem J. 1966 Jul;100(1):63-8.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Frequently asked questions
How should I store and thaw Premium Fetal Horse Serum (P-FHS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
Related products
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- Fetal Bovine Serum, Horse Serums
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