- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Lipid-Depleted Fetal Horse Serum (LD-FHS)
Fetal horse serum with lipids removed, for studying serum’s role without interference from undefined lipids.
- Removes the wide range of undefined lipid species found in serum
- For lipid-metabolism and lipid-sensitive research
Description
Background
Since serum is usually present in cell culture studies, its lipid metabolism makes studying the role of serum in cultured cells very challenging. That is because serum contains a wide range of undefined lipid species. PurMa™ Lipid-Depleted Fetal Horse Serum (LD-FHS) performs as the highest level of lipid depletion of serum . Lipid-Depleted Fetal Horse Serum (LD-FHS) performs crucial roles in:
- Where the normal concentration of lipids may interfere with the results.
- It is also crucial for studies directly involving lipid metabolism. Because the composition of serum can have a profound impact on important cellular checkpoints.
- Moreover, LD-FHS performs in investigations about the role of lipids in their corresponding intracellular pathways.
- Also, it acts to generate the media with defined lipid species.
PurMa Biologics uses a cutting-edge methodology to remove lipids from serum without eliminating its ability to support the proliferation of cells in culture.
Our proprietary method includes
- Applying isopropyl ether and butanol with a special ratio.
- PurMa Biologics has spent many years determining the needed density of ether and butanol as well as the ratio of these two solvents in this process to gain the best result.
- 4X washed, each for 3 hours, with isopropyl ether-butanol with decreasing amounts of butanol in every washing, in a way that the last two washes are carried out without butanol.
- Dialyzing with a 3500 Dalton dialysis tube for 4 days with low salts physiological buffer at physiological pH.
- To prepare 500 ml Lipid-Depleted Fetal Horse Serum (LD-FHS), 10 changes of 10 liters of buffer is needed.
- The dialysis will be terminated when the buffers show no isopropanol using GC–MS.
- The serum goes through a vigorous analysis for pathogens as well as growth and MTT (proliferation ability) using 2 cell lines. For complete details, please see below: quality control as well as “Analysis Tab”).
- The resulting high-quality serum lacks most of lipids and can still be used to support the growth and maintenance of many cell lines in culture.
- USE NOT LESS THAN 15% SERUM IN YOUR MEDIA
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
Special Features
- PurMa™Lipid-Depleted Fetal Horse Serum (LD-FHS) is of USA origin.
- We sterilize the serums with gamma irradiation.
- PurMa™ LD-FHS has been heat inactivated.
- PurMa™ Lipid-Depleted Fetal Horse Serum (LD-FHS) has been depleted of lipids and dialyzed using the 3500 Dalton dialysis tube.
- Is free of lipids, phospholipids, and a long chain of fatty acids
- Has been vigorously analyzed with the certificate of analysis.
- Free of Mycoplasma, IgG, steroids and hormones, and other pathogens.
- Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
1 review for Lipid-Depleted Fetal Horse Serum (LD-FHS)
Frequently asked questions
How should I store and thaw Lipid-Depleted Fetal Horse Serum (LD-FHS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
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Verified Customer –
I used Lipid Free horse serum of PurMa Biologics.
The quality is great!