Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
Fetal bovine serum with endotoxin reduced to very low levels for endotoxin-sensitive work.
- Triple filtered at 0.1 µm, then processed through PurMa’s Endotoxin Elimination Column
- Each lot evaluated for endotoxin
- Ideal where residual endotoxin can interfere with results
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
PurMa Biologics manufactures seven types of bovine serum, three of which, however, are considered premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover, We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm, the residual endotoxin was depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).
Not sure which serum fits your cells? See our complete guide to cell culture serum.
PurMaTM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
- PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- Lastly, we vigorously analyze various aspects and each LOT possesses a certificate of analysis.
Endotoxin Removal Procedure
- PurMa Biologics processes PurMaTM ED-FBS from PurMaTM Premium Fetal Bovine Serum (P-FBS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
- The process of removing endotoxin from FBS routinely starts with passing the premium FBS through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) at the rate of 0.1 ml/minute.
- The unbound fractions pass through 0.1µm filters three times. The mentioned procedure eliminates the residual of endotoxin as well.
- We conjugate Sepharose 6B in our proprietary column covalently to a powerful endotoxin-specific absorbent.
Analysis
PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Biochemical and morphological studies of steady state and lipopolysaccharide treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
- The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | Not Detected | Not Detected |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing (9 CFR 113.53c) | |||
| Bovine Adenovirus | NA | Not Detected | Not Detected |
| Bovine Parvovirus | NA | Not Detected | Not Detected |
| Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
| Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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