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Advanced MEM Alpha Modification
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SKU: Select options below to see it Categories: Cell Culture Media, MEM Alpha Modification in Earle’s Buffer (MEM-α) Tags: Cell Culture, Earle's Buffer, EBSS, glucose, MEM, MEM Alpha, MEM-α, Phenol Red, Sodium Bicarbonate, Standard, Without Phenol Red

MEM Alpha Modification in Earle’s Buffer (MEM-α)

MEM Alpha Modification in Earle’s Buffer (MEM-α) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. Scientist around the globe use MEM -α Modification for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN)

SKU Select options below to see it
SKU: N/A Categories: Cell Culture Media, MEM Alpha Modification in Earle’s Buffer (MEM-α) Tags: Cell Culture, Earle's Buffer, EBSS, glucose, MEM, MEM Alpha, MEM-α, Phenol Red, Sodium Bicarbonate, Standard, Without Phenol Red

Description

Background

MEM Alpha Modification in Earle’s Buffer (MEM-α) is certainly a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

MEM Alpha Modification in Earle’s Buffer (MEM-α)  contains

  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.
  • Also, MEM Alpha Modification in Earle’s Buffer (MEM-α) does not contain deoxyribonucleosides and ribonucleosides.
  • Moreover, PurMaTM MEM-α media in Earle’s buffer includes Earles’ buffer

The composition of Earle’s buffer, it contains:

  1. Calcium chloride
  2. Magnesium sulfate
  3. Potassium chloride
  4. Sodium bicarbonate
  5. Sodium chloride
  6. Sodium Phosphate, monobasic

Additionally, MEM Alpha Modification in Earle’s Buffer (MEM-α)

it contains:

  • Low level of glucose (1.00 g/ liter)
  • More importantly, Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
  • 2 mM L-glutamine (292 mg/L)
  • Also, PurMa Biologics manufactures 118 types of MEM-α

Application

This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, MEM Alpha Modification in Earle’s Buffer (MEM-α) has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Formulation

Complete formulation is in the ” Formulation tab” as well as here: MEM-alpha Formulation in Earle’s Buffer

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay et al.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Additional information

Condition

MEM-α Standard Formulation, MEM-α w/o Glutamine, MEM-α w/o Phenol Red, MEM-α w/o Sodium Bicarbonate, MEM-α w/o Glucose, MEM-α w/o Lipoic Acid, MEM-α w/o Glutamine, w/o Phenol Red, MEM-α w/o Glutamine, w/o Sodium Bicarbonate, MEM-α w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α 15 mM HEPES (3.6 g/L), MEM-α 25 mM HEPES (5.9 g/L), MEM-α w/o Sodium Pyruvate, MEM-α High Glucose (4.5 g/L), MEM-α High Sodium Bicarbonate (3.7 g/L), MEM-α Low Sodium Bicarbonate (0.85 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

★★★★★
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
Ghazal Rastegar UT Dallas
★★★★★
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Gregory Mullen OU Health Sciences Center
★★★★★
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Haus Bio Verified customer
★★★★★
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Verified Customer
★★★★★
Very fast, efficient, and economic service. Great for new PIs like me.
Surendra Shukla OU Health Sciences Center
★★★★★
it is reliable vendor!
Jianhua Song Applied StemCell

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Frequently asked questions

How should I store MEM Alpha Modification in Earle’s Buffer (MEM-α)?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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